1887

Abstract

SUMMARY

A canine virus derived from a diseased dog has been plaque-purified and characterized in detail. Analysis of infected cells demonstrated that virus antigen accumulated in the nucleus at 12 to 24 h post-infection and the cytopathology at the ultrastructural level was diagnostic of a parvovirus infection. The purified virus particles were 23 to 26 nm in diam. and banded at a density 1.44 g/ml in CsCl. Detailed biochemical analysis revealed a single-stranded DNA genome and three structural proteins of mol. wt. 82300, 67300 and 63500. All of the data presented are consistent with the classification of this virus as a parvovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-1-113
1982-09-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/1/JV0620010113.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-1-113&mimeType=html&fmt=ahah

References

  1. Appel M. J., Cooper B. J., Greisen H., Scott F., Carmichael L. E. 1979a; Canine viral enteritis. 1. Status report on corona- and parvo-like viral enteritides. Cornell Veterinarian 69:123–133
    [Google Scholar]
  2. Appel M. J., Scott F. W., Carmichael L. E. 1979b; Isolation and immunization studies of a canine parvo-like virus from dogs with haemorrhagic enteritis. Veterinary Record 105:156–159
    [Google Scholar]
  3. Binn L. N., Lazar E. C., Eddy G. A., Kajima M. 1970; Recovery and characterization of a minute virus of canines. Infection and Immunity 1:503–508
    [Google Scholar]
  4. Carmichael L. E., Joubert J. C., Pollock R. V. 1980; Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. American Journal of Veterinary Research 40:784–791
    [Google Scholar]
  5. Carpenter J. L., Roberts R. M., Harpster N. K., King N. W. 1980; Intestinal and cardiopulmonary forms of parvovirus infection in a litter of pups. Journal of the American Veterinary Medical Association 176:1269–1273
    [Google Scholar]
  6. Else R. W. 1980; Fetal haemorrhagic enteritis in a puppy associated with a parvovirus infection. Veterinary Record 106:14–15
    [Google Scholar]
  7. Gagnon A. N., Povey R. C. 1979; A possible parvovirus associated with an epidemic gastroenteritis of dogs in Canada. Veterinary Record 104:263–264
    [Google Scholar]
  8. Handa H., Shiroki K., Shimojo H. 1978 Expression of helper function for adeno-associated virus in adenovirus transformed cells. In Replication of Mammalian Parvoviruses pp. 99–107 Edited by Ward D., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Hayes M. A., Russel R. G., Babiuk L. A. 1979; Sudden death in young dogs with mycocarditis caused by parvovirus. Journal of the American Veterinary Medical Association 174:1197–1203
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:467–490
    [Google Scholar]
  11. Johnson R. H., Spradbrow P. B. 1979; Isolation from dogs with severe enteritis of a parvovirus related to feline panleukopenia virus. Australian Veterinary Journal 55:151
    [Google Scholar]
  12. Johnson F. B., Blacklow M. R., Hoggan M. D. 1972; Immunological reactivity of antisera prepared against the sodium dodecyl sulfate-treated structural polypeptide of adenovirus-associated virus. Journal of Virology 9:1017–1026
    [Google Scholar]
  13. Karasaki S. 1966; Size and ultrastructure of the H-viruses as determined with the use of specific antibodies. Journal of Ultrastructure Research 16:109–122
    [Google Scholar]
  14. Kongsvik J., Hopkins M., Ellem K. 1978 Two populations of infectious virus produced during H-l infection of synchronized transformed cells. In Replication of Mammalian Parvoviruses pp. 505–520 Edited by Ward D., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and l4C on polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  17. Lenghaus C., Studdert M. J. 1980; Relationship of canine panleukopaenia (enteritis) and myocarditis parvoviruses to feline panleucopaenia virus. Australian Veterinary Journal 56:152–153
    [Google Scholar]
  18. Mcmaster G., Beard P., Engers H., Hirt B. 1981a; Characterization of an immunosuppressive parvovirus related to minute virus of mice. Journal of Virology 38:317–326
    [Google Scholar]
  19. Mcmaster G., Tratschin J., Siegl G. 1981b; Comparison of canine parvovirus with mink enteritis virus by restriction site mapping. Journal of Virology 38:368–371
    [Google Scholar]
  20. Paradiso P. R. 1981; The infectious process of the parvovirus H-1: correlation of protein content, particle density and viral infectivity. Journal of Virology 39:800–807
    [Google Scholar]
  21. Peterson J., Dale R., Karess R., Leonard D., Ward D. 1978 Comparison of parvovirus structural proteins: evidence for post-translational modification. In Replication of Mammalian Parvoviruses pp. 431–446 Edited by Ward D., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–219
    [Google Scholar]
  23. Rhode S. L. 1982; Complementation for replicative form DNA of a deletion mutant of H-l by various parvoviruses. Journal of Virology (in press)
    [Google Scholar]
  24. Robinson W. F., Huxtable C. R., Pass D. A. 1980; Canine parvoviral myocarditis: a morphologic description of the natural disease. Veterinary Pathology 17:282–293
    [Google Scholar]
  25. Rose J. 1974 Parvovirus reproduction. In Comprehensive Virology vol. 3 pp. 1–62 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  26. Siegl G., Kronauer G. 1980; A plaque assay for feline panleukopenia virus. Journal of General Virology 46:211–218
    [Google Scholar]
  27. Singer I. 1974 The intracellular localization of parvovirus (H-l) antigens using immunocytochrome C. In Viral Immunodiagnosis pp. 101–123 Edited by Kurstak E. New York: Academic Press;
    [Google Scholar]
  28. Singer I. I. 1975; Ultrastructural studies of H-l parvovirus replication. II. Induced changes in the deoxyribonucleoprotein and ribonucleoprotein components of human NB cell nuclei. Experimental Cell Research 95:205–217
    [Google Scholar]
  29. Singer I. I. 1976; Ultrastructural studies of H-1 parvovirus replication. III. Intracellular localization of viral antigens with immunocytochrome C. Experimental Cell Research 99:346–356
    [Google Scholar]
  30. Singer I. I., Rhode S. L. 1977; Ultrastructural studies of H-l parvovirus replication. IV. Crystal development and structure with the temperature-sensitive mutant tsl. Journal of Virology 24:343–352
    [Google Scholar]
  31. Singer I., Toolan H. 1975; Ultrastructural studies of H-l parvovirus replication. I. Cycopathology produced in human NB cells and hamster embryo fibroblasts. Virology 65:40–54
    [Google Scholar]
  32. Tattersall P. 1978 Parvovirus protein structure and virion maturation. In Replication of Mammalian Parvoviruses pp. 53–72 Edited by Ward D., TattersallD P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Wilson N. D. 1980; Origin of canine parvovirus. Veterinary Record 106: p. 392
    [Google Scholar]
  34. Zechel K., Weber K. 1977; Degradation of nucleic acids in cell lysates by SI nuclease in the presence of 9 M urea and sodium dodecylsulfate. European Journal of Biochemistry 77:133–139
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-1-113
Loading
/content/journal/jgv/10.1099/0022-1317-62-1-113
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error