1887

Abstract

SUMMARY

Virus particles and virus proteins excreted from cultured human embryonic lung () cells infected with varicella-zoster virus () were examined by electron microscopy and affinity column chromatography using an antibody to followed by SDS-polyacrylamide gel electrophoresis (). Approximately 1 × 10 to 2 × 10 virus particles/ml with no detectable infectivity, of which 30 to 80% were enveloped, were observed in the culture fluid 48 to 72 h after infection, when cytopathic effect (c.p.e.) appeared. In the sonicated infected cell suspension, 1 × 10 to 2 × 10 virus particles/ml, of which 30 to 50% were enveloped, were observed and the virus particle/infectivity ratio was approx. 10:1. The culture fluid of infected HEL cells labelled with [S]methionine or [H]glucosamine was centrifuged at 100000 g for 2 h to remove virus particles and the supernatant was examined for excreted virus proteins. Affinity column chromatography of the supernatant using immobilized human zoster convalescent serum, led to the isolation of virus antigens which were analysed by SDS-PAGE. Polypeptides with mol. wt. of approx. 115K and 45K, both of which were glycosylated, were detected, suggesting that these glycoproteins were excreted from the infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-2-271
1982-08-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/2/JV0610020271.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-2-271&mimeType=html&fmt=ahah

References

  1. Achong B. G., Meurisse E. v. 1968; Observation on the fine structure and replication of varicella virus in cultivated human amnion cells. Journal of General Virology 3:305–308
    [Google Scholar]
  2. Asano Y., Takahashi M. 1978; Studies on neutralization of varicella-zoster virus and serological follow-up of cases of varicella and zoster. Biken Journal 21:15–23
    [Google Scholar]
  3. Asano Y., Shiraki K., Takahashi M., Nagai H., Ozaki T., Yazaki T. 1981; Soluble skin test antigen-of varicella-zoster virus prepared from the fluid of infected cultures. Journal of Infectious Diseases 143:684–692
    [Google Scholar]
  4. Becker p., Melnick J. L., Mayor H. D. 1965; A morphological comparison between the developmental stages of herpes zoster and human cytomegalovirus. Experimental and Molecular Pathology 4:11–23
    [Google Scholar]
  5. Cook M. L., Stevens J. G. 1968; Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture. Journal of Virology 2:1458–1464
    [Google Scholar]
  6. Gershon A., Cosio L., Brunell p. A. 1973; Observations on the growth of varicella-zoster virus in human diploid cells. Journal of General Virology 18:21–31
    [Google Scholar]
  7. Iltis J. p., Oakes J. E., Hyman R. w., Rapp R. 1977; Comparison of the DNAs of varicella-zoster viruses isolated from clinical cases of varicella and herpes zoster. Virology 82:345–352
    [Google Scholar]
  8. Kaplan A. s., Erickson J. s., Ben-Porat T. 1975; Synthesis of proteins in cells infected with herpes virus. X. Proteins excreted by cells infected with herpes simplex virus type 1 and 2. Virology 64:132–143
    [Google Scholar]
  9. Martin M. L., Palmer E. L. 1973; Complement-fixing antigens produced by varicella-zoster virus in tissue culture. Applied Microbiology 26:410–413
    [Google Scholar]
  10. Norrild B., Vestergaad B. F. 1979; Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. Intervirology 11:104–110
    [Google Scholar]
  11. Pennington T. H., Mccrae M. A. 1977; Processing of a pseudorabies virus-induced protein which is glycosylated, sulphated and excreted. Journal of General Virology 34:155–165
    [Google Scholar]
  12. Randall R. E., Killington R. A., Watson D. H. 1980; Glycosylations with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus type 1 and 2. Journal of General Virology 48:297–310
    [Google Scholar]
  13. Shiraki K., Okuno T., Yamanishi K., Takahashi M. 1982; Polypeptides of varicella-zoster virus (VZV) and immunological relationship of VZV and herpes simplex virus (HSV). Journal of General Virology 61:255–269
    [Google Scholar]
  14. Svedmyr A. 1965; Varicella virus in HeLa cells. Archiv für die gesamte Virusforschung 17:495–503
    [Google Scholar]
  15. Takahashi M., Okuno Y., Otsuka T., Osame J., Takamizawa A., Sasada T., Kubo T. 1975; Development of a live attenuated varicella vaccine. Biken Journal 18:25–33
    [Google Scholar]
  16. Taylor-Robinson D., Downie A. w. 1959; Chickenpox and herpes zoster. 1. Complement fixation studies. British Journal of Experimental Pathology 40:398–409
    [Google Scholar]
  17. Watson D. H., Russel W. c., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250–260
    [Google Scholar]
  18. Weller T. H., Witton H. M. 1958; The etiologie agents of varicella and herpes zoster. Serologic studies with the viruses as propagated in vitro. Journal of Experimental Medicine 108:869–890
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-61-2-271
Loading
/content/journal/jgv/10.1099/0022-1317-61-2-271
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error