Six cell fusion-causing syn mutants were isolated from the KOS (syn-101 to syn-106) and three from the HFEM (syn-107 to syn-109) strains of herpes simplex virus type 1 (HSV-1). The mutants were studied by complementation and recombination with syn-20 (a syncytial mutant of KOS) and ts-B5 (a syncytial mutant of HFEM). Some studies also employed MP, a syncytium-inducing strain isolated from the non-syncytial parent, mP. Complementation and recombination of syn-20 and ts-B5 indicated that these two mutants were altered in two different virus genes. The recombination frequency between syn-20 and ts-B5 was very similar to that observed between MP and ts-B5, indicating that syn-20 and MP may represent alterations in the same virus gene, syn-101, syn-103, syn-104 and syn-105 were tentatively assigned to the syn-20 complementation group, while syn-107 and syn-109 were tentatively assigned to the fs-B5 complementation group, syn-106 and syn-108 were excluded from the ts-B5 group, syn-102 could not be excluded from either complementation group, syn-101 induced markedly less fusion at 38 °C relative to 34 °C. At 34 °C the patterns of syn-101-infected cell peptides and glycopeptides, examined by SDS-gel electrophoresis, were normal, but at 38 °C the amount of glycopeptide gC was particularly reduced, syn-102 produced decreased amounts of glycoproteins, and a non-glycosylated peptide, probably ICP6, was absent from extracts infected with syn-106.
BrownS. M.,
RitchieD. H.,
Subak SharpeJ. H.1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recom- bination analysis leading to a linkage map. Journal of General Virology 18:329–346
HonessR. W.,
RoizmanB.1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes simplex polypeptides in the infected cell. Journal of Virology 12:1347–1365
HonessR. W.,
BuchanA.,
HalliburtonI. W.,
WatsonD. H.1980; Recombination and linkage between structural and regulatory genes of herpes simplex virus type 1 : study of the functional organization of the genome. Journal of Virology 34:716–742
KnowlesR. W.,
Persons.1976; Effects of 2-deoxyglucose, glucosamine, and mannose on cell fusion and the glycoproteins of herpes simplex virus. Journal of Virology 18:644–651
LittleS. P.,
SchafferP. A.1981; Expression of the syncytial (syn) phenotype in HSV-1, strain KOS: genetic and phenotypic studies of mutants in two syn loci. Virology 112:686–702
ManservigiR.,
SpearP. G.,
BuchanA.1977; Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins. Proceedings of the National Academy of Sciences of the United States of America 74:3913–3917
PersonS.,
KnowlesR. W.,
ReadG. S.,
WarnerS. C.,
BondV. C.1976; Kinetics of cell fusion induced by a syncytia-producing mutant of herpes simplex virus type 1. Journal of Virology 17:183–190
PizerL. I.,
CohénG. H.,
EisenbergR. J.1980; Effect of tunicamycin on herpes simplex virus glycoproteins and infectious virus production. Journal of Virology 34:142–153
RuyechanW. T.,
MorseL. S.,
KnipeD. M.,
RoizmanB.1979; Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of genetic loci specifying the social behavior of infected cells. Journal of Virology 29:677–697
SchafferP. A.,
TevethiaM. J.,
Benyesh-MelnickM.1974; Recombination between temperature-sensitive mutants of herpes simplex virus type 1. Virology 58:219–228
YamamotoS.,
KabutaH.1976; Genetic analysis of polykaryocytosis by herpes simplex virus. II. Recombination between viruses with non-fusing and fusing ability. Kurume Medical Journal 23:209–217
YamamotoS.,
KabutaH.1977; Genetic analysis of polykaryocytosis by herpes simplex virus. III. Complementation and recombination between non-fusing mutants and construction of a linkage map with regard to the fusion function. Kurume Medical Journal 24:162–172