1887

Abstract

SUMMARY

Epizootic haemorrhagic disease virus () was seen by light and electron microscopy to replicate in perinuclear locations. Tubules, paracrystals and virus matrices were associated with replication sites. As infection proceeded, aggregates of virus migrated towards the cell periphery, resulting in cell membrane rupture near the virus aggregate with the subsequent release of the virus aggregates. Virus release, as seen by light microscopy, gave the appearance of occurring by a ‘budding’ process whereby part of the cell would swell and subsequently rupture or break away. Infectivity studies indicated that approx. 80% of newly replicated virus was released extracellularly in aggregates which required disruption to maximize infectious virus yield. Trypsin did not enhance virus infectivity. Of the six isolates used in this study each isolate was characterized by its own maximum yield obtained after several serial passages in cell culture.

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1982-08-01
2021-10-25
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References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin On the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  3. Bando B. M. 1975; Isolation of bluetongue and epizootic hemorrhagic disease viruses in cell culture. Proceedings of the Eighteenth Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians163–174
    [Google Scholar]
  4. Esparza J., Gorziglia M., Gil F., Römer H. 1980; Multiplication of human rotavirus in cultured cells: an electron microscope study. Journal of General Virology 47:461–472
    [Google Scholar]
  5. Fosberg S. A., Stäuber E. H., Renshaw H. W. 1977; Isolation and characterization of epizootic hemorrhagic disease virus from white-tailed deer (Odocoileus virginianus) in eastern Washington. American Journal of Veterinary Research 38:361–364
    [Google Scholar]
  6. Foster N. M., Breckon R. D., Luedke A. J., Jones R. H., Metcalf H. E. 1977; Transmission of two strains of epizootic hemorrhagic disease virus in deer by Culicoides variipennis. Journal of Wildlife Diseases 13:9–16
    [Google Scholar]
  7. Graham D. Y., Estes M. K. 1980; Proteolytic enhancement of rotavirus infectivity: biology mechanism. Virology 101:432–439
    [Google Scholar]
  8. Huismans H., Els H. J. 1979; Characterization of the microtubules associated with the replication of three different orbiviruses. Virology 92397–406
    [Google Scholar]
  9. Huismans H., Bremer C. W., Barber T. L. 1979; The nucleic acid and proteins of epizootic haemorrhagic disease virus. Onderstepoort Journal of Veterinary Research 46:95–103
    [Google Scholar]
  10. Humason G. L. 1962 Animal Tissue Techniques pp. 130–133 San Francisco: W. H. Freeman and Company;
    [Google Scholar]
  11. Jochim M. M., Barber T. L., Bando B. M. 1974; Identification of bluetongue and epizootic hemorrhagic disease viruses by the indirect fluorescent antibody procedure. Proceedings of the Seventeenth Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians91–103
    [Google Scholar]
  12. Joklik W. K. 1974 Reproduction of Reoviridae. In Comprehensive Virology vol. 2 pp. 231–334 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  13. Kontor E. J., Welch A. B. 1976; Characterization of an epizootic hemorrhagic disease virus. Research in Veterinary Science 21:190–196
    [Google Scholar]
  14. Lawman M. J. P., Gibbs E. P. J., Davies J. A. 1977; Epizootic haemorrhagic disease of deer virus in tissue culture and laboratory animals. Journal of Comparative Pathology 87:345–352
    [Google Scholar]
  15. Murphy F. A., Borden E. C., Shope R. E., Harrison A. 1971; Physiochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus—a new taxonomic group. Electron microscopic studies. Journal of General Virology 13:273–288
    [Google Scholar]
  16. Spendlove R. S., Mcclain M. E. 1967; Enhancement of reovirus infectivity by capsid removal. Bacteriological Proceedings 20:135
    [Google Scholar]
  17. Spendlove R. S., Mcclain M. E. 1968 Enhancement and inactivation of reovirus infectivity by proteolytic enzymes. In Medical and Applied Virology pp. 127–130 Edited by Sanders M., Lennette E. H. St. Louis: W. H. Green;
    [Google Scholar]
  18. Spendlove R. S., Mcclain M. E., Lennette E. H. 1970; Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes. Journal of General Virology 8:83–94
    [Google Scholar]
  19. Svehag S. E., Leendersten L., Gorham J. R. 1966; Sensitivity of bluetongue virus to lipid solvents, trypsin and pH changes and its serological relationship to arboviruses. Journal of Hygiene 64:339–346
    [Google Scholar]
  20. Thomas F. C., Miller J. 1971; A comparison of bluetongue virus and EHD virus. Electronmicroscopy and serology. Canadian Journal of Comparative Medicine 35:22–27
    [Google Scholar]
  21. Tsai K., Karstad L. 1970; Epizootic hemorrhagic disease virus of deer: an electron microscopic study. Canadian Journal of Microbiology 16:427–432
    [Google Scholar]
  22. Tsai K., Karstad L. 1973; The pathogenesis of epizootic hemmorrhagic disease of deer: an electron microscopic study. American Journal of Pathology 70:379–391
    [Google Scholar]
  23. Verwoerd D. W., Huismans H., Erasmus B. J. 1979 Orbiviruses. In Comprehensive Virology vol. 14 pp. 285–345 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  24. Von Bertalanffy Masin M., Masin F. 1958; A new and rapid method for diagnosis of vaginal and cervical cancer by fluorescence microscopy. Cancer 11:873–887
    [Google Scholar]
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