1887

Abstract

SUMMARY

A persistent infection with Saint Louis encephalitis () virus in a poikilothermic cell line TH-1 (turtle heart cells) was studied. Infected TH-1 cells were subcultured weekly at 31 °C for 1 year and continued to produce low levels (10 to 10 p.f.u./ml) of virus without obvious cytopathic effects or marked cyclic events. Indirect fluorescent antibody and infectious centre assays indicated that < 1 % of the cells were producing detectable virus proteins or infectious virus. Defective-interfering particles, temperature-sensitive mutants and provirus were not detected. Interferon () mediation of the persistent infection was considered since the persistently infected cells () and normal TH-1 cells were resistant to heterologous virus challenge after treatment with virus-free culture fluid from . A direct relationship was found between the m.o.i. and the amount of produced, plateauing at an m.o.i. of approx. 10. The reptilian IFN was physically and chemically similar to mammalian and avian . Certain biological markers of the virus changed during the persistent infection. It was less virulent for mice, showed distinct differences in cell culture host range and had increased thermal lability.

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1982-08-01
2024-04-25
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References

  1. Bellanti J. A. (editor) 1978 Mechanisms of immunity to viral diseases. In Immunology vol. 2 pp. 385–417 Philadelphia: W. B. Sanders Co;
    [Google Scholar]
  2. Clark H. F., Karzon D. T. 1967 a; Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene Carolina. Experimental Cell Research 48:263–268
    [Google Scholar]
  3. Clark H. F., Karzon D. T. 1967 b; Acquired tolerance to elevated temperatures in a poikilothermic cell line (terrapene heart, TH-1). Experimental Cell Research 48:269–275
    [Google Scholar]
  4. Eaton B. T. 1979; Heterologous interference in Aedes albopictus cells infected with alphaviruses. Journal oj Virology 30:45–55
    [Google Scholar]
  5. Engelking H. M., Leong J. C. 1981; IHNV persistently infects Chinook salmon embryo cells. Virology 109:47–58
    [Google Scholar]
  6. Falcoff E., Fauconnier B. 1965; In vitro production of an interferon-like inhibitor of viral multiplication by a poikilothermic animal cell (Testudo greca). Proceedings of the Society for Experimental Biology and Medicine 18:609–612
    [Google Scholar]
  7. Fleischman W. R. Jun, Simon E. H. 1974; Mechanism of interferon induction by NDV: a monolayer and single cell study. Journal of General Virology 25:337–349
    [Google Scholar]
  8. Friedman R. M. 1977; Antiviral activity of interferons. Bacteriological Reviews 41:543–567
    [Google Scholar]
  9. Friedman R. M., Ramseur J. M. 1979; Mechanisms of persistent infections by cytopathic viruses in tissue culture. Archives of Virology 60:83–103
    [Google Scholar]
  10. Galabov A., Savov Z., Vassileva V. 1973; Interferon production in arbovirus-infected cell cultures of tortoise (Testudo graeca) kidney. Acta virologica 17:1–10
    [Google Scholar]
  11. Grahm A. F. 1977 Possible role of defective virus in persistent infection. In Microbiology pp. 445–450 Edited by Schlessinger D. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  12. Haase A. T., Stowring L., Ventura P., Traynot B., Johnson K., Swoveland P., Smith M., Britten-darnall M., Faras A., Narayan O. 1977 Role of DNA intermediates in persistent infections caused by RNA viruses. In Microbiology pp. 478–483 Edited by Schlessinger D. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  13. Holland J. J., Villarreal L. D. 1974; Persistent noncytocidal vesicular stomatitis viral infections mediated by defective particles that suppress virion transcriptase. Proceedings of the National Academy of Sciences of the United States of America 71:2956–2960
    [Google Scholar]
  14. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–332
    [Google Scholar]
  15. Igarashi A. 1979; Characteristics of Aedes albopictus cells persistently infected with dengue viruses. Nature, London 280:690–691
    [Google Scholar]
  16. Igarashi A., Koo R., Stollar V. 1977; Evolution and properties of Aedes albopictus cell cultures persistently infected with Sindbis virus. Virology 82:69–83
    [Google Scholar]
  17. Kawai A., Matsumoto S., Tanabe K. 1975; Characterization of rabies virus recovery from persistently infected BHK cells. Virology 67:520–523
    [Google Scholar]
  18. Lyerla H. C., Forrester F. T. 1979; Immuno-fluorescence methods in virology. Center for Disease Control U.S. Department of Health, Education and Welfare
    [Google Scholar]
  19. Marcus P. I., Sekellick M. J., Fuller F. J. 1978; Double-stranded RNA: the interferon inducer of viruses. Fourth International Congress for Virology (abstracts) P. 107
    [Google Scholar]
  20. Meinkoth J., Kennedy S. I. T. 1980; Semliki Forest virus persistence in mouse L929 cells. Virology 100:141–155
    [Google Scholar]
  21. Monath T. P., Cropp C. B., Bowen G. S., Kemp G. E., Mitchell C. J., Gardner J. J. 1980; Variation in virulence for mice and rhesus monkeys among St. Louis encephalitis virus strains of different origin. American Journal of Tropical Medicine and Hygiene 29:948–962
    [Google Scholar]
  22. Nishiyama Y. 1977; Studies of L cells persistently infected with VSV: factors involved in the resultation of persistent infection. Journal of General Virology 35:265–279
    [Google Scholar]
  23. Pedersen F. S., Hazeltine W. A. 1980; Analysis of the genome of an endogenous ecotrophic retrovirus of the AKR strain of mice. Journal of Virology 33:349–365
    [Google Scholar]
  24. Preble O. T., Youngner J. S. 1975; Temperature-sensitive viruses and the etiology of chronic and inapparent infections. Journal of Infectious Diseases 131:467–473
    [Google Scholar]
  25. Ramseur J. M., Friedman R. M. 1977; Prolonged infection of interferon-treated cells by vesicular stomatitis virus: possible role of temperature-sensitive mutants and interferon. Journal of General Virology 37:523–533
    [Google Scholar]
  26. Reeves W. C. 1974; Overwintering of arboviruses. Progress in Medical Virology 17:193–220
    [Google Scholar]
  27. Riedel B., Brown D. T. 1977; Role of extracellular virus in the maintenance of the persistent infection induced by Aedes albopictus (mosquito) cells by Sindbis virus. Journal of Virology 23:554–561
    [Google Scholar]
  28. Schmaljohn C. S., Blair C. D. 1977; Persistent infection of cultured mammalian cells by Japanese encephalitis virus. Journal of Virology 24:580–589
    [Google Scholar]
  29. Schmaljohn C. S., Blair C. D. 1979; Clonal analysis of mammalian cell cultures persistently infected with Japanese encephalitis virus. Journal of Virology 31:816–822
    [Google Scholar]
  30. Sekellick M. J., Marcus D. I. 1980; The interferon system as a regulator of persistent infection. Annals of the New York Academy of Sciences 350:545–557
    [Google Scholar]
  31. Stancek D. 1965; The role of interferon in tick-borne encephalitis virus-infected L-cells. III. The effects of temperature on the production of virus and interferon by L-cells during acute and persistent infection. Acta virologica 9:298–307
    [Google Scholar]
  32. Tew J. G., Burmeister J., Greene E. J., Pflaumer S. K., Goldstein J. 1977; A radioimmunoassay for human antibody specific for microbial antigens. Journal of Immunological Methods 14:231–241
    [Google Scholar]
  33. Thomas L. A., Ecklund C. M. 1960; Overwintering of western equine encephalomyelitis virus in experimentally infected garter snakes and transmission to mosquitoes. Proceedings of the Society for Experimental Biology and Medicine 105:52–55
    [Google Scholar]
  34. Walker D. L. 1964; The viral carrier state in animal cell cultures. Progress in Medical Virology 6:111–148
    [Google Scholar]
  35. Weiss B., Rosenthal R., Schlesinger S. 1980; Establishment and maintenance of persistent infection by Sindbis virus in BHK cells. Journal of Virology 33:463–474
    [Google Scholar]
  36. Wiktor T. J., Clark H. F. 1972; Chronic rabies infection in cell cultures. Infection and Immunity 6:988–995
    [Google Scholar]
  37. Zhdanov V. M., Parfanovich M. I. 1974; Integration of measles virus nucleic acid into the cell genome. Archiv fur die gesamte Virusforschung 45:225–234
    [Google Scholar]
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