Genetic and Antigenic Variation in Type 3 Polioviruses: Characterization of Strains by Monoclonal Antibodies and T1 Oligonucleotide Mapping Free

Abstract

SUMMARY

Considerable genetic and antigenic heterogeneity was detected among a collection of 17 poliovirus type 3 strains isolated between 1939 and 1958 in studies using monoclonal antibodies and by T1 oligonucleotide mapping. Heterogeneity was detected even amongst a collection of nine viruses designated Saukett and assumed to originate from the same prototype virus. The monoclonal antibodies were found to differ in their strain specificities for poliovirus type 3 strains in virus neutralization or single-radial-immunodiffusion tests. Relationships between strains detected in this way were in general consistent with those detected by oligonucleotide mapping. One of the monoclonal antibodies (NIBp 56) was able to distinguish between certain Saukett virus strains which differed by as little as a single specific oligonucleotide. The heterogeneity detected amongst Saukett viruses is of potential practical importance since these strains are used widely in the manufacture of inactivated poliovirus vaccine.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-2-167
1982-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/2/JV0610020167.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-2-167&mimeType=html&fmt=ahah

References

  1. Clewley J. P., Bishop D. H. L., Kang C. Y., Coffin J., Schnitzlein W. M., Reichmann M. E., Shope R. E. 1977; Oligonucleotide finger prints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup. Journal of Virology 23:152–166
    [Google Scholar]
  2. Dömök I., Magrath D. I. 1979; Guide to poliovirus isolation and serological techniques for poliomyelitis surveillance. WHO Offset Publications No 46:
    [Google Scholar]
  3. Ferguson M., Schild G. C., Minor P. D., Yates P. J., Spitz M. 1981; A hybridoma cell line secreting antibody to poliovirus type 3 ‘D’ antigen: detection in virus harvest of two ‘D’ antigen populations. Journal of General Virology 54:437–443
    [Google Scholar]
  4. Galfre G., Milstein C., Wright B. 1979; Rat X rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature, London 277:131–133
    [Google Scholar]
  5. Gerhard W., Webster R. G. 1978; Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR8/34 (H0N1) influenza virus with monoclonal antibodies. Journal of Experimental Medicine 148:382–392
    [Google Scholar]
  6. Harris T. J. R., Robson K. J. H., Brown F. 1980; A study of the level of nucleotide sequence conservation between the RNAs of two serotypes of foot and mouth disease virus. Journal of General Virology 50:403–418
    [Google Scholar]
  7. Kohler G., Milstein C. 1975; Continuous culture of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  8. Koprowski H. 1980 Monoclonal hybridomas as a new tool in immunology. In Microbiology pp. 191–195 Edited by Schlessinger D. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  9. Koprowski H., Gerhard W., Croce C. M. 1977; Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proceedings of the National Academy of Sciences of the United States of America 74:2985–2988
    [Google Scholar]
  10. Lubeck M. D., Schulman J. L., Palese P. 1980; Antigenic variants of influenza viruses: marked differences in the frequencies of variants selected with different monoclonal antibodies. Virology 102:458–462
    [Google Scholar]
  11. Mcbride W. D. 1959; Antigenic analysis of polioviruses by kinetic studies of serum neutralization. Virology 1:45–58
    [Google Scholar]
  12. Minor P. D. 1980; Comparative biochemical studies of type 3 polioviruses. Journal of Virology 34:73–84
    [Google Scholar]
  13. Minor P. D. 1982; Characterization of strains of type 3 poliovirus by oligonucleotide mapping. Journal of General Virology 59:307–317
    [Google Scholar]
  14. Nottay B. K., Kew O. M., Hatch M. H., Heyward J. T., Obueski J. F. 1981; Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans. Virology 108:405–423
    [Google Scholar]
  15. Salk J., Bennet B. L., Lewis L. J., Ward E. N., Youngner U. S. 1953; Studies in human subjects on active immunization against poliomyelitis. I. A preliminary report of experiments in progress. Journal of the American Medical Association 151:1081–1098
    [Google Scholar]
  16. Schild G. C., Wood J. M., Minor P. D., Dandawate C. N., Magrath D. I. 1980; Immunoassay of poliovirus antigens by single radial diffusion: development and characteristics of a sensitive autoradiographic zone size enhancement (ZE) technique. Journal of General Virology 51:157–170
    [Google Scholar]
  17. Van Wezel A. L., Hazendonk A. G. 1979; Intratypic serodifferentiation of poliomyelitis strains by strain specific antisera. Intervirology 11:2–8
    [Google Scholar]
  18. Webster R. G., Laver W. G. 1980; Determination of the number of non-overlapping antigenic areas on Hong Kong (H3N2) influenza virus haemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology 104:139–148
    [Google Scholar]
  19. Wiktor T. J., Koprowski H. 1978; Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants. Proceedings of the National Academy of Sciences of the United States of America 75:3938–3942
    [Google Scholar]
  20. Yewdell J. W., Webster R. G., Gerhard W. 1979; Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature, London 279:246–251
    [Google Scholar]
  21. Young J. F., Desselberger U., Palese P. 1979; Evolution of human influenza A viruses in nature: sequential mutations in the genomes of new H1N1 isolates. Cell 18:73–83
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-61-2-167
Loading
/content/journal/jgv/10.1099/0022-1317-61-2-167
Loading

Data & Media loading...

Most cited Most Cited RSS feed