1887

Abstract

SUMMARY

We have generated several variants of the human lymphoid cell line Namalwa which were characterized as high producers, non-producers and spontaneous producers of interferon (IFN). All of them were stable for more than 1 year, suggesting chromosomal inheritance of their mutations. We show the kinetics and quantity of IFN production following treatment with several inducers, including polyriboinosinic-polyribocytidylic acid [poly(rI).poly(rC)].

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1982-07-01
2024-12-05
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References

  1. Armstrong J. A. 1971; Semi-micro dye binding assay for rabbit interferon. Applied Microbiology 21:723–726
    [Google Scholar]
  2. Bridgen P. J., Anfinsen C. B., Corley L., Bose S., Zoon K. C., Ruegg V. T., Buckler C. E. 1977; Human lymphoblastoid interferon: large scale production and partial purification. Journal of Biological Chemistry 252:6585–6587
    [Google Scholar]
  3. De Clercq E. 1974; Synthetic interferon inducers. Current Topics in Chemistry 52:173–208
    [Google Scholar]
  4. Havell E. A., VILCEK J. 1972; Production of high titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemjournalapy 2:476–484
    [Google Scholar]
  5. Iscove N. N., Schreier M. H. 1979 Clonal growth of cells in semi-solid or viscous medium. In Immunological Methods pp. 379–385 Edited by Lefkovits I., Pernis B. New York: Academic Press;
    [Google Scholar]
  6. Kohase M., Vilcek J. 1977; Regulation of human interferon production stimulated with poly (I), poly (C). Correlation between shutoff and hyporesponsiveness to reinduction. Virology 76:47–54
    [Google Scholar]
  7. Pickering L. A., Kronenberg L. H., Stewart W. E. II 1980; Spontaneous production of human interferon. Proceedings of the National Academy of Sciences of the United States of America 77:5938–5942
    [Google Scholar]
  8. Thompson L. H., Mankovitch R., Baker R. M., Till J. E., Siminovitch L., Whitemore G. F. 1970; Isolation of temperature-sensitive mutants of L-cells. Proceedings of the National Academy of Sciences of the United States of America 66:377–384
    [Google Scholar]
  9. Tovey M. G., Begon-Lours J., Gresser I, MORRIS A. G. 1977; Marked enhancement of interferon production in 5-BdU-treated human lymphoblastoid cells. Nature, London 267:455–456
    [Google Scholar]
  10. Trapman J. 1979; A systematic study of interferon production by mouse L-929 cells induced with poly(I).poly(C) and DEAE-dextran. FEBS Letters 98:107–110
    [Google Scholar]
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