@article{mbs:/content/journal/jgv/10.1099/0022-1317-61-1-83, author = "Frixen, Uwe and von der Helm, Klaus and Friis, Robert and Wille, Wolfgang and Willecke, Klaus", title = "Fusion Injection of Rous Sarcoma Virus Proteins into Rous Sarcoma Virus-transformed, Non-producing Hamster Cells Causes Release of Infectious Virus", journal= "Journal of General Virology", year = "1982", volume = "61", number = "1", pages = "83-86", doi = "https://doi.org/10.1099/0022-1317-61-1-83", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-61-1-83", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "hamster cells", keywords = "RSV", keywords = "protein microinjection", keywords = "td mutants", abstract = "SUMMARY Purified virus proteins from transformation-defective (td) mutants of Rous sarcoma virus PrA or PrB were trapped in human erythrocyte ghosts which, after resealing, were fusion-injected into hamster RBH cells or rat TWERC cells. These cell lines are non-productively transformed by subgroup C Rous sarcoma virus. After fusion injection the hamster RBH cells released transforming subgroup C Rous sarcoma virus. No infectious virus could be rescued from rat TWERC cells. Since previous experiments have shown that fusion injection of the purified Rous sarcoma virus protein p 15 into hamster RBH cells caused cleavage of the precursor protein pr76 to form the virus group-specific antigen (gag) but did not induce infectious virus, we conclude that in addition to p 15 other virus proteins are required to induce virus rescue in hamster RBH cells.", }