1887

Abstract

SUMMARY

Purified virus proteins from transformation-defective () mutants of Rous sarcoma virus PrA or PrB were trapped in human erythrocyte ghosts which, after resealing, were fusion-injected into hamster cells or rat cells. These cell lines are non-productively transformed by subgroup C Rous sarcoma virus. After fusion injection the hamster RBH cells released transforming subgroup C Rous sarcoma virus. No infectious virus could be rescued from rat cells. Since previous experiments have shown that fusion injection of the purified Rous sarcoma virus protein p 15 into hamster RBH cells caused cleavage of the precursor protein pr76 to form the virus group-specific antigen (gag) but did not induce infectious virus, we conclude that in addition to p 15 other virus proteins are required to induce virus rescue in hamster cells.

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/content/journal/jgv/10.1099/0022-1317-61-1-83
1982-07-01
2021-10-21
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References

  1. Aupoix M., Vigier P. 1976; Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses. International Journal of Cancer 18:787–797
    [Google Scholar]
  2. Eisenman R., Vogt V., Diggelmann H. 1974; Synthesis of avian RNA tumor virus structural protein. Cold Spring Harbor Symposia on Quantitative Biology 39:1067–1075
    [Google Scholar]
  3. Simcovic D. 1972; Characteristics of tumors induced in mammals, especially rodents, by viruses of the avian leukosis sarcoma group. Advances in Virus Research 17:95–127
    [Google Scholar]
  4. Steimer K. S., Boettger D. 1977; Complementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion. Journal of Virology 23:133–141
    [Google Scholar]
  5. Svec J., Svec F., Simkovic D., Thurzo V. 1970; Induction of tumors in hamsters by rat Rous sarcoma RBI, producing chicken sarcoma virus. I. Continuous production of virus oncogenic for chicks by hamster sarcoma cells. Journal of the National Cancer Institute 44:521–527
    [Google Scholar]
  6. Svoboda J., Hlozanek J. 1970; Role of cell association in virus infection and virus rescue. Advances in Cancer Research 13:217–269
    [Google Scholar]
  7. Svoboda J., Machala O., Donner L., Sovova V. 1971; Comparative study of RSV rescue from RSV-transformed mammalian cells. International Journal of Cancer 8:391–400
    [Google Scholar]
  8. Svoboda J., Popovic M., Sainerova H., Mach O., Shoyab M., Baluda M. A. 1977; Incomplete viral genome in a non-virogenic mouse tumour cell line (RVP3) transformed by Prague strain of avian sarcoma virus. International Journal of Cancer 19:851–858
    [Google Scholar]
  9. Vigier P. 1972; Persistence of Rous sarcoma virus in transformed non-permissive cells: mechanism of virus induction by association with permissive cells in the absence of Sendai virus. International Journal of Cancer 9:150–161
    [Google Scholar]
  10. Von der helm K., Kempeni J., Wille W., Willecke K. 1980; Rous sarcoma virus precursor protein pr76 is processed in avian sarcoma virus-transformed mammalian cells after fusion injection of viral protein p 15. Virology 106:310–316
    [Google Scholar]
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