1887

Journal of General Virology header logo

Volume 61, Issue 1

Serological Characterization of C-type Retroviruses Endogenous to the C57BL/6 Mouse and Isolated in Tumours Induced by Radiation Leukaemia Virus (RadLV-Rs) Free

Abstract

SUMMARY

Radiation leukaemia virus, Rs strain (RadLV-Rs), is a virus complex derived from radiation-induced lymphoma of the C57BL/6 mouse. Several B-ecotropic retroviruses (T1223/B, T98/B and T128/B) were isolated from the RadLV-Rs and further cloned. They were found to be highly leukaemogenic and polymorphic in terms of XC cell fusion activity. To investigate a possible relationship between their phenotypical and genotypical properties, a serological characterization of their proteins was undertaken by means of interference, neutralization and type-specific radioimmunological experiments. In addition, these viruses were compared on the basis of the electrophoretic mobility of their proteins. They were also compared with the prototype endogenous N-ecotropic (BL6/NÌ) and xenotropic (T530/X) retroviruses of the C57BL/6 mouse as well as the AKR-MuLV and the RadLV/VL-3 (thymotropic leukaemogenic retrovirus involved in radio-induced lymphoma). With respect to the pl2, T1223/B and T128/B viruses were of xenotropic type as in RadLV/VL-3, whereas T98/B p12 displayed ecotropic and xenotropic type-specific antigenic determinants. The gp71 of all B-ecotropic virus isolates were indistinguishable and of the AKR-MuLV type. This latter result was further supported by interference and neutralization experiments. This supports the view that the B-ecotropic virus isolates originated by recombination (one or two events) between N-ecotropic and xenotropic endogenous retroviruses. The RadLV/ VL-3 possesses a unique envelope recombinant glycoprotein of which the antigenicity was not observed in the RadLV-Rs complex. Thus, it may be assumed that the leukaemogenic components of RadLV and RadLV-Rs arose by different recombinational mechanisms.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 6 mouse , B-tropism , C57BL , leukaemogenic retroviruses and protein
© Society for General Microbiology 1982
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-1-55
1982-07-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/1/JV0610010055.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-1-55&mimeType=html&fmt=ahah

References

  1. Barbacid M., Stephenson J. R., Aaronson S. A. 1975; gaggene of mammalian type C RNA tumour viruses. Nature, London 262:554–559
     [Google Scholar]
  2. Bassin R. H., Tuttle N., Fischinger P. J. 1970; Isolation of murine sarcoma virus transformed mouse cells which are negative for leukemia virus from agar suspension cultures. International Journal of Cancer 6:97–107
     [Google Scholar]
  3. Bassin R. H., Tuttle N., Fischinger P. J. 1971; Rapid cell culture assay technique for murine leukaemia viruses. Nature, London 229:564–566
     [Google Scholar]
  4. Benade L. E., Ihle J. N. 1980; Different serotypes of B-tropic murine leukemia viruses and association with endogenous ecotropic viral loci. Virology 106:374–386
     [Google Scholar]
  5. Benade L. E., Ihle J. N., Decleve A. 1978; Serological characterization of B-tropic viruses of C57BL mice: possible origin by recombination of endogenous N-tropic and xenotropic viruses. Proceedings of the National Academy of Sciences of the United States of America 75:4553–4557
     [Google Scholar]
  6. Decleve A., Lieberman M., Ihle J. N., Kaplan H. S. 1976; Biological and serological characterization of radiation leukemia virus. Proceedings of the National Academy of Sciences of the United States of America 73:4675–4679
     [Google Scholar]
  7. Decleve A., Lieberman M., Ihle J. N., Rosenthal P. N., Lung M. L., Kaplan H. s. 1978; Physicochemical, biological and serological properties of a leukemogenic virus isolated from cultured RadLV-induced lymphomas of C57BL/Ka mice. Virology 90:23–35
     [Google Scholar]
  8. Devare S. G., Stephenson J. R. 1977; Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. Journal of Virology 23:443–447
     [Google Scholar]
  9. Fischinger P. J., Nomura S., Peebles P. T., Haapala D. K., Bassin R. H. 1972; Reversion of murine sarcoma virus transformed mouse cells: variants without a rescuable sarcoma virus. Science 176:1033–1035
     [Google Scholar]
  10. Gautsch J. W., Elder J. H., Schindler J., Jensen F. C., Lerner R. A. 1978; Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism. Proceedings of the National Academy of Sciences of the United States of America 75:4170–4174
     [Google Scholar]
  11. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of131I-Labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
     [Google Scholar]
  12. Guillemain B., Astier T., Mamoun R., Duplan J. F. 1980; In vitro production and titration assays of B-tropic retroviruses isolated from C57BL mouse tumours induced by radiation leukemia virus (RadLV-Rs): effect of dexamethasone. Intervirology 13:65–73
     [Google Scholar]
  13. Hartley J. W., Rowe W. P., Huebner R. J. 1970; Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. Journal of Virology 5:221–225
     [Google Scholar]
  14. Jainchill J. L., Aaronson S. A., Todaro G. J. 1969; Murine sarcoma and leukemia viruses: assay using clonal lines of contact inhibited mouse cells. Journal of Virology 4:549–553
     [Google Scholar]
  15. Janowski M., Astier T., Guillemain B., Baugnet-mahieu L., Duplan J. F., Maisin J. 1978; Characterization by molecular hybridization of two viral populations derived from a radiation leukemia virus. Cancer Research 38:3487–3493
     [Google Scholar]
  16. Latarjet R., Duplan J. F. 1962; Experiment and discussion on leukemogenesis by cell-free extracts of radiation-induced leukemia in mice. International Journal of Radiation Biology 5:339–344
     [Google Scholar]
  17. Legrand E., Daculsi R., Duplan J. F. 1981; Characteristics of the cell populations involved in extra-thymic lymphosarcoma induced in C57BL/6 mice by RadLV-Rs. Leukemia Research 5:223–233
     [Google Scholar]
  18. Lieber M. M., Sherr C. J., Todaro G. J. 1974; S-tropic murine type C viruses: frequency of isolation from continuous cell lines, leukemic virus preparations and normal spleens. International Journal of Cancer 13:587–598
     [Google Scholar]
  19. Lieberman M., Kaplan H. S. 1959; Leukemogenic activity of filtrates from radiation-induced lymphoid tumours of mice. Science 30:387–388
     [Google Scholar]
  20. Lonai P., Haran-ghera N. 1980; Genetic resistance to murine leukemia induced by different radiation leukemia virus variants: a comparative study on the role of the H-2 complex. Immunogenetics 11:21–25
     [Google Scholar]
  21. Mamoun R., Guillemain B., Astier T., Portail J. P., Legrand E., Duplan J. F. 1978; Production et analyse d'un complexe viral derive d'un virus des radioleucoses de la souris C57BL. International Journal of Cancer 22:98–105
     [Google Scholar]
  22. Mamoun R. Z., Astier T., Guillemain B. 1981; Establishment and propagation of a bovine leukaemia virus-producing cell line derived from the leukocytes of a leukaemic cow. Journal of General Virology 54:357–365
     [Google Scholar]
  23. Mistry P. B., Duplan J. F. 1973; Proprietes biologiques d'un virus isole d'une radioleucemie C57BL. I. Premiers passages du virus natif. Bulletin du Cancer 60:287–300
     [Google Scholar]
  24. Pincus T., Hartley J. W., Rowe W. P. 1971; A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. Journal of Experimental Medicine 133:1219–1233
     [Google Scholar]
  25. Stephenson J. R., Aaronson S. A., Arnstein P., Huebner R. J., Tronick S. R. 1974; Demonstration of two immunologically distinct xenotropic type C RNA viruses of mouse cells. Virology 61:56–63
     [Google Scholar]
  26. Strand M., August J. T. 1976; Oncornavirus envelope glycoprotein in serum of mice. Virology 75:130–144
     [Google Scholar]
  27. Svoboda J. 1961; The tumorigenic action of Rous sarcoma in rats and the permanent production of Rous virus by the induced rat sarcoma XC. Folia Biologica(Praha) 7:46–60
     [Google Scholar]
  28. Todaro G. J., Green H. 1963; Quantitative studies of the growth of mouse cells in culture and their development into established cell lines. Journal oj Cellular Biology 17:299–313
     [Google Scholar]
  29. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-61-1-55
Loading
Serological Characterization of C-type Retroviruses Endogenous to the C57BL/6 Mouse and Isolated in Tumours Induced by Radiation Leukaemia Virus (RadLV-Rs)
J. Gen. Virol. 61, 55 (1982); https://doi.org/10.1099/0022-1317-61-1-55
/content/journal/jgv/10.1099/0022-1317-61-1-55
/content/journal/jgv/10.1099/0022-1317-61-1-55
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error