1887

Abstract

SUMMARY

Peripheral blood lymphocytes of chickens bearing tumours induced by avian sarcoma virus can be specifically stimulated to divide by the crude culture fluids of virus-infected cells. In this communication, we show that relevant antigenic activity apparently resides in each of the internal virus proteins p15 and p27. The ability of infectious culture fluids to be mitogenic for sensitized lymphocytes is greatly reduced following treatment with antibodies specific for either total avian myeloblastosis virus (AMV) protein or for p27.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-60-2-391
1982-06-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/60/2/JV0600020391.html?itemId=/content/journal/jgv/10.1099/0022-1317-60-2-391&mimeType=html&fmt=ahah

References

  1. Boyum A. 1968; Separation of leucocytes from blood and bone marrow. Scandinavian Journal of Clinical Investigation 21: (Supplement B) 9–15
    [Google Scholar]
  2. Green R. W., Bolognesi D. P. 1974; Isolation of proteins by gel filtration in 6 M guanidine hydrochloride: application to RNA tumor viruses. Analytical Biochemistry 57:108–117
    [Google Scholar]
  3. Hall M. R., Qualtiere L. F., Meyers P. 1979; Cellular and humoral immune reactivity to tumor-associated antigens in chickens infected with Rous sarcoma virus. Journal of Immunology 123:1097–1105
    [Google Scholar]
  4. Israel E., Wainberg M. A. 1977; Development of cellular anti-tumor immunity in chickens bearing tumors induced by Rous sarcoma virus. Journal of Immunology 118:2237–2242
    [Google Scholar]
  5. Lee J. C., Ihle J. N. 1979; Mechanism of C-type viral leukemogenesis. 1. Correlation of in vitro lymphocyte blastogenesis to viremia and leukemia. Journal of Immunology 123:2351–2358
    [Google Scholar]
  6. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  7. Oppermann H., Bishop J. M., Varmus H. E., Levin Ow L. 1977; A joint product of the genes gag and pol of avian sarcoma virus: a possible precursor of reverse transcriptase. Cell 12:993–1005
    [Google Scholar]
  8. Temin H. M., Rubin H. 1958; Characteristics of an assay for Rous sarcoma cells in tissue culture. Virology 6:669–688
    [Google Scholar]
  9. Van Eldik L. J., Paulson J. C., Green R. W., Smith R. E. 1978; The influence of carbohydrate on the antigenicity of the envelope glycoprotein of avian myeloblastosis virus and B77 avian sarcoma virus. Virology 86:193–202
    [Google Scholar]
  10. Vogt P. K., Hu S. S. F. 1977; The genetic structure of RNA tumor viruses. Annual Review of Genetics 11:203–238
    [Google Scholar]
  11. Wainberg M. A., Israel E. 1978; Enhancement of avian sarcoma virus-induced tumor growth after pre-treatment with BCG. Infection and Immunity 22:328–333
    [Google Scholar]
  12. Wainberg M. A., Israel E., Schwartz-Luft E., Yu M. 1977; Differential expression of relevant Rous sarcoma-associated antigens in cultured cells. Cancer Research 37:3026–3033
    [Google Scholar]
  13. Wainberg M. A., Yu M., Israel E. 1979a; Decreased production of transforming virus and altered antigenic behaviour in cultured avian sarcoma cells. Journal of General Virology 42:255–264
    [Google Scholar]
  14. Wainberg M. A., Beiss B., Wahi R., Israel E. 1979b; Thymic dependence of cell-mediated immunity to avian sarcomas in chickens. Cellular Immunology 45:344–355
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-60-2-391
Loading
/content/journal/jgv/10.1099/0022-1317-60-2-391
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error