1887

Abstract

SUMMARY

Formalin-fixed strain Cowan, bearing protein A, routinely used for the absorption of antigen-antibody complexes, was found to bind protein kinase activity from disrupted Moloney murine leukaemia virus (Mo-MuLV). The Wood strain of lacking protein A also bound the kinase with similar efficiency. About 50% of the bound kinase activity, as detected by phosphorylation of casein using [-P]ATP, could be eluted from the bacterial preparation with buffer containing 0.5 -KCl. Similar results were obtained with Moloney murine sarcoma virus (Mo-MuSV) strain 349 and 110 MuSV(MuLV). The bacterial preparation was also found to bind casein kinase activity from cellular extracts of uninfected, Rauscher murine leukaemia virus (R-MuLV)-infected and Mo-MuLV-infected cells. Analysis of [H]leucine-labelled proteins from purified virus showed selective binding to of only two major labelled virus proteins. One virus component bound to had the relative mobility of p15; the other polypeptide co-migrated with virus p10. Upon exposure to increased salt concentration, most of the p10 but very little of the p15 proteins were released. The -binding proteins from 110 Mo-MuSV and MuSV-349 revealed similar binding and elution patterns of p10 and p15 molecules. The p10 and protein kinase activity eluted from Mo-MuLV-absorbed bacteria were separated by gel filtration into a high molecular weight species, containing p10 and kinase activity, and a low molecular weight p10 monomer lacking enzymic activity.

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/content/journal/jgv/10.1099/0022-1317-60-2-365
1982-06-01
2022-01-22
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