1887

Abstract

SUMMARY

Electron microscopic analyses have revealed that the bottom (B) component of cowpea mosaic virus alone induces cytopathic structures in cowpea mesophyll protoplasts, similar to those induced by the complete virus [i.e. B plus middle (M) components]. This indicates that the development of such structures is not linked to accumulation of virus particles but to virus RNA replication and expression. When purified B component was inoculated to primary cowpea leaves, symptoms were not produced and B component RNA was incapable of spreading to surrounding cells. The results are discussed in terms of limits to the independence of B component RNA and of possible functions for M component RNA-encoded proteins.

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1982-06-01
2024-11-14
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References

  1. Assink A. M., Swaans H., Van Kammen A. 1973; The localization of virus.specific double.stranded RNA of cowpea mosaic virus in subcellular fractions of infected Vigna leaves. Virology 53:384–391
    [Google Scholar]
  2. Bruening G. 1977; Plant covirus systems: two component systems. In Comprehensive Virology vol 11: pp 55–141 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  3. Cadman C. H. 1962; Evidence for association of tobacco rattle virus nucleic acid with a cell component. Nature, London 193:49
    [Google Scholar]
  4. De Jager C. P. 1976; Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests. Virology 70:151–163
    [Google Scholar]
  5. De Zoeten G. A., Assink A. M., Van Kammen A. 1974; Association of cowpea mosaic virus induced double.stranded RNA with a cytopathic structure in infected cells. Virology 59:341–355
    [Google Scholar]
  6. Franssen H., Goldbach R., Broekhuusen M., Moerman M., Van Kammen A. 1982; Expression of middle component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom component RNA.encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  7. Goldbach R. W., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B.component RNA of cowpea mosaic virus. Nature, London 286:297–300
    [Google Scholar]
  8. Goldbach R. W., Schilthuis J. G., Rezelman G. 1981; Comparison of in vivo and in vitro translation of cowpea mosaic virus RNAs. Biochemical and Biophysical Research Communications 99:89–95
    [Google Scholar]
  9. Goldbach R., Rezelman G., Zabel P., Van Kammen A. 1982; Expression of the bottom component RNA of cowpea mosaic virus: evidence that the 60 kilodalton VPg.precursor is cleaved into single VPg and a 58 kilodalton polypeptide. Journal of Virology (in press)
    [Google Scholar]
  10. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–315
    [Google Scholar]
  11. Kushida H. 1961; A new embedding method for ultrathin sectioning using a methacrylate resin with three dimensional polymer structure. Journal of Electron Microscopy 10:194–197
    [Google Scholar]
  12. Lister R. M. 1968; Functional relationship between virus.specific products of infection by viruses of the tobacco rattle type. Journal of General Virology 2:43–58
    [Google Scholar]
  13. Lister R. M. 1969; Tobacco rattle NETU, virus in relation to functional heterogeneity of plant viruses. Federation Proceedings 28:1875–1889
    [Google Scholar]
  14. Pelham H. R. B. 1979; Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology 96:463–477
    [Google Scholar]
  15. Reunders L., Aalbers A. M. J., Van kammen A., Thuring R. W. J. 1974; Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions. Virology 60:515–521
    [Google Scholar]
  16. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  17. Rezelman G., Goldbach R. W., Van Kammen A. 1980; Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. Journal of Virology 36:366–373
    [Google Scholar]
  18. Rottier P. J. M., Rezelman G., Van kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  19. Sanger H. L., Brandenburg E. 1961; Uber die Gewinnung von Infektiosem aus ‘Wintertyp’. Pflanzen des Tabak. Rattle. Virus durch Phenolextraktion. Naturwissenschaften 48:391
    [Google Scholar]
  20. Stanley J., Goldbach R. W., Van Kammen A. 1980; The genome.linked protein of cowpea mosaic virus is coded by RNA from the bottom component. Virology 106:180–182
    [Google Scholar]
  21. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:635–642
    [Google Scholar]
  22. Van Kammen A. 1968; The relationship between the components of cowpea mosaic virus. I. Two ribonucelo. protein particles necessary for the infectivity of CPMV. Virology 34:312–318
    [Google Scholar]
  23. Van Kammen A. 1972; Plant viruses with a divided genome. Annual Review of Phytopathology 10:125–150
    [Google Scholar]
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