%0 Journal Article %A Stinski, Mark F. %A Thomsen, Darrell R. %A Rodriguez, Jose E. %T Synthesis of Human Cytomegalovirus-specified RNA and Protein in Interferon-treated Cells at Early Times After Infection %D 1982 %J Journal of General Virology, %V 60 %N 2 %P 261-270 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-60-2-261 %K interferon %K cytomegalovirus %K RNA %K protein %I Microbiology Society, %X SUMMARY In human fibroblast cells treated with interferon, cytomegalovirus-specified immediate early RNA was found associated with the polyribosomes at concentrations and size classes similar to the virus RNA found in non-treated cells. Interferon treatment inhibited the translation of the immediate early virus mRNA; the relative rate of virus-specified immediate early protein and antigen synthesis decreased with increasing concentrations of interferon. In addition, the relative amount of virus-specified RNA associated with the polyribosomes at early times after infection was significantly reduced by treatment of the cells with interferon. Inhibition of infectious virus production in interferon-treated cells was primarily due to inhibition of immediate early virus protein synthesis and secondarily to suppression of early virus RNA synthesis. The role of the virus-specified immediate early proteins in regulating subsequent virus gene expression is discussed. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-60-2-261