@article{mbs:/content/journal/jgv/10.1099/0022-1317-60-2-261, author = "Stinski, Mark F. and Thomsen, Darrell R. and Rodriguez, Jose E.", title = "Synthesis of Human Cytomegalovirus-specified RNA and Protein in Interferon-treated Cells at Early Times After Infection", journal= "Journal of General Virology", year = "1982", volume = "60", number = "2", pages = "261-270", doi = "https://doi.org/10.1099/0022-1317-60-2-261", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-60-2-261", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "interferon", keywords = "cytomegalovirus", keywords = "RNA", keywords = "protein", abstract = "SUMMARY In human fibroblast cells treated with interferon, cytomegalovirus-specified immediate early RNA was found associated with the polyribosomes at concentrations and size classes similar to the virus RNA found in non-treated cells. Interferon treatment inhibited the translation of the immediate early virus mRNA; the relative rate of virus-specified immediate early protein and antigen synthesis decreased with increasing concentrations of interferon. In addition, the relative amount of virus-specified RNA associated with the polyribosomes at early times after infection was significantly reduced by treatment of the cells with interferon. Inhibition of infectious virus production in interferon-treated cells was primarily due to inhibition of immediate early virus protein synthesis and secondarily to suppression of early virus RNA synthesis. The role of the virus-specified immediate early proteins in regulating subsequent virus gene expression is discussed.", }