1887

Abstract

SUMMARY

Uncoating of influenza virus (strain WSN) in MDCK cells was studied by following the fate of the virus labelled with radioactive precursors. The accumulation of subviral components of input virus was observed in nuclear-associated cytoplasm (NAC) obtained by treatment of the nuclei with citric acid. Two types of subviral components were found there, ribonucleoproteins (RNPs) and larger subviral particles (SVP) containing RNPs in association with M protein. SVP, with different relative amounts of M protein, were revealed in NAC, suggesting that M protein was gradually released from RNPs. The released RNPs entered the nuclei while M protein accumulated within perinuclear membranes. Thus, SVP could be regarded as probable intermediates in virus uncoating. Rimantadine prevented the release of M protein from RNPs and their penetration into the nuclei provoking the accumulation of subviral components in NAC.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-60-1-49
1982-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/60/1/JV0600010049.html?itemId=/content/journal/jgv/10.1099/0022-1317-60-1-49&mimeType=html&fmt=ahah

References

  1. Bukrinskaya A. G., Vorkunova N. K. 1979; Association of influenza virus parental nucleocapsids with chromatin in infected cell nuclei. Voprosy Virusologii 2:186–187
    [Google Scholar]
  2. Bukrinskaya A. G., Vorkunova G. K., Vorkunova N. K. 1979; Cytoplasmic and nuclear input virus RNPs in influenza virus-infected cells. Journal of General Virology 45:557–567
    [Google Scholar]
  3. Bukrinskaya A. G., Vorkunova N. K., Pushkarskaya N. L. 1982; Uncoating of a rimantadine-resistant variant of influenza virus in the presence of rimantadine. Journal of General Virology 60:61–66
    [Google Scholar]
  4. Dourmashkin R. R., Tyrrell D. A. 1974; Electron microscopic observations on the entry of influenza virus into susceptible cells. Journal of General Virology 24:129–141
    [Google Scholar]
  5. Hay A. J., Kennedy N. C. T., Skehel J. J., Appleyard G. 1979; The matrix protein gene determines amantadine-sensitivity of influenza virus. Journal of General Virology 42:189–191
    [Google Scholar]
  6. Helenius A., Marsh M., White J. 1980; The entry of viruses into animal cells. Trends in Biochemical Sciences 5:104–106
    [Google Scholar]
  7. Huang R. T. C., Wahn K., Klenk H. D., Rott R. 1980; Fusion between cell membrane and liposomes containing the glycoproteins of influenza virus. Virology 104:294–302
    [Google Scholar]
  8. Hudson J. B., Flawith J., Dimmock N. J. 1978; Early events in influenza virus infection. III. The formation of a nucleoplasmic ribonucleoprotein complex from the input virion. Virology 87:167–176
    [Google Scholar]
  9. Kato N., Eggers H. J. 1969; Inhibition of uncoating of fowl plague virus by 1-adamantanamine hydrochloride. Virology 37:632–641
    [Google Scholar]
  10. Koff W. C., Knight V. 1979; Inhibition of influenza virus uncoating by rimantadine hydrochloride. Journal of Virology 31:261–263
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lazarowitz S., Goldberg A. R., Choppin P. W. 1973; Proteolytic cleavage by plasmin of the HA polypeptide of influenza virus. Host cell activation of serum plasminogen. Journal of Virology 56:172–180
    [Google Scholar]
  13. Lenard J. 1978; Virus envelopes and plasma membranes. Annual Review of Biophvsics and Bioengineering 7:139–165
    [Google Scholar]
  14. Lubeck M. D., Schulman J. L., Palese P. 1978; Susceptibility of influenza A viruses to amantadine is influenced by the gene coding for M protein. Journal of Virology 28:710–716
    [Google Scholar]
  15. Morgan C., Rose H. M. 1968; Structure and development of viruses as observed in the electron microscope. VIII. Entry of influenza virus. Journal of Virology 2:925–936
    [Google Scholar]
  16. Novikoff A. B., Novikoff P. 1977; Cytochemical contributions to differentiating GERL from Golgi apparatus. Histochemical Journal 9:525–551
    [Google Scholar]
  17. Ro-Choi T. S., Smetana K., Busch H. 1973; Citric acid sonication method for the isolation of nucleoli. Experimental Cell Research 79:43–52
    [Google Scholar]
  18. Schulze J. T. 1972; The structure of influenza virus. II. A model based on the morphology and composition of subviral particles. Virology 47:181–196
    [Google Scholar]
  19. Skehel J. J., Hay A. J., Armstrong J. A. 1977; On the mechanism of inhibition of influenza virus replication by amantadine hydrochloride. Journal of General Virology 38:97–110
    [Google Scholar]
  20. Stephenson J. R., Dimmock N. J. 1975; Early events in influenza virus multiplication. I. Location and fate of the input RNA. Virology 65:77–86
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-60-1-49
Loading
/content/journal/jgv/10.1099/0022-1317-60-1-49
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error