Production of High Titres of Interferon-γ by Cells Derived from Short-term Cultures of Murine Spleen Leukocytes in T-Cell Growth Factor-conditioned Medium Free

Abstract

SUMMARY

Short-term culture of activated murine spleen cells in T-cell growth factor-conditioned medium resulted in a cell population that produced high titres of interferon-γ in response to the T-cell mitogen concanavalin A. Compared to interferon-γ production by freshly isolated spleen cells in response to the same mitogen, titres were 10 to 20 times higher at all cell densities tested. Furthermore, the minimal cell density at which interferon-γ production could be detected was significantly lower. The mitogen dose required for optimal interferon induction in this system was higher than in conventional spleen cell cultures.

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/content/journal/jgv/10.1099/0022-1317-60-1-195
1982-05-01
2024-03-28
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References

  1. Berthold W., Tan C., Tan Y. H. 1978; Purification and in vitro labeling of interferon from a human fibroblastoid cell line. Journal of Biological Chemistry 253:5206–5212
    [Google Scholar]
  2. De Maeyer-Guignard J., Tovey M. G., Gresser I., Demaeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly(U)- and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  3. Interferon Nomenclature 1980 Nature, London 286:110
    [Google Scholar]
  4. Langford M. P., Georgiades G. J., Dianzani F., Johnson H. M. 1979; Large-scale production and physicochemical characterization of human immune interferon. Infection and Immunity 26:36–41
    [Google Scholar]
  5. Marcucci F., Waller M., Kirchner H., Krammer P. 1981; Production of immune interferon by murine T-cell clones from long-term cultures. Nature, London 291:79–81
    [Google Scholar]
  6. Rubinstein M., Rubinstein S., Familletti P. C., Miller R. S., Waldmann A., Pestka S. 1978; Human leucocyte interferon purified to homogeneity. Science 202:1289–1290
    [Google Scholar]
  7. Wheelock E. F. 1965; Interferon-like virus inhibitor induced in human leucocytes by phytohemagglutinin. Science 149:310–311
    [Google Scholar]
  8. Wietzerbin J., Stefanos S., Lucero M., Falcoff E., O’malley J., Sulkowski E. 1979; Physicochemical characterization and partial purification of mouse immune interferon. Journal of General Virology 44:773–781
    [Google Scholar]
  9. Yip Y. K., Pang H. L., Urban C., Vilcek J. 1981; Partial purification and characterization of human (immune) interferon. Proceedings of the National Academy of Sciences of the United States of America 78:1601–1605
    [Google Scholar]
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