1887

Abstract

SUMMARY

The O45 (V) mutant of vesicular stomatitis virus (VSV) has been used to demonstrate that discrimination between positive (+) and negative (-) strand nucleocapsids in the process of budding mature virions is maintained despite the lack of detectable G protein in either the plasma membrane or the virus membrane. These data indicate that the VSV G protein plays no active role in the discrimination between negative- and positive-strand nucleocapsids which will mature to virions. The synthesis of intracellular nucleocapsids in O45 (V)-infected cells at permissive and non-permissive conditions was also examined in comparison with that of the wild-type virus. No differences were observed in intracellular nucleocapsid quantity or polarity in any of these cases despite a 97% inhibition of virus yield in the case of O45 (V) at non-permissive temperature.

Keyword(s): glycoprotein , nucleocapsid , RNA and VSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-60-1-165
1982-05-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/60/1/JV0600010165.html?itemId=/content/journal/jgv/10.1099/0022-1317-60-1-165&mimeType=html&fmt=ahah

References

  1. Cartwright B. 1977; Effect of concanavalin A on vesicular stomatitis virus maturation. Journal of General Virology 34:249–256
    [Google Scholar]
  2. Hunt L. A., Summers D. F. 1976; Association of vesicular stomatitis virus proteins with HeLa cell membranes and released viruses. Journal of Virology 20:637–645
    [Google Scholar]
  3. Knipe D. M., Baltimore D., Lodish H. F. 1977a; Separate pathways of maturation of the major structural proteins of vesicular stomatitis virus. Journal of Virology 21:1128–1139
    [Google Scholar]
  4. Knipe D. M., Baltimore D., Lodish H. F. 1977b; Maturation of viral proteins in cells infected with temperature-sensitive mutants of vesicular stomatitis virus. Journal of Virology 21:1149–1159
    [Google Scholar]
  5. Knipe D. M., Lodish H. F., Baltimore D. 1977c; Localization of two cellular forms of the vesicular stomatitis viral glycoprotein. Journal of Virology 21:1121–1127
    [Google Scholar]
  6. Lafay F. 1974; Envelope proteins of vesicular stomatitis virus: effect of temperature sensitive mutations in complementation groups III and V. Journal of Virology 14:1220–1228
    [Google Scholar]
  7. Little S. P., Huang A. S. 1977; Synthesis and distribution of vesicular stomatitis virus-specific polypeptides in the absence of progeny production. Virology 81:37–47
    [Google Scholar]
  8. Martinet C., Combard A., Printz-Ane C., Printz P. 1979; Envelope proteins and replication of vesicular stomatitis virus: in vivo effects of RNA + temperature-sensitive mutations on viral RNA synthesis. Journal of Virology 29:123–133
    [Google Scholar]
  9. Morrison T. G., Mcquain C. O. 1978; Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes. Journal of Virology 26:115–125
    [Google Scholar]
  10. Rothman J. E., Katz F. M., Lodish H. F. 1978; Glycosylation of a membrane protein is restricted to the growing polypeptide chain but is not necessary for insertion as a transmembrane protein. Cell 15:1447–1454
    [Google Scholar]
  11. Schmidt M. F. G., Schlesinger M. J. 1979; Fatty acid binding to vesicular stomatitis virus glycoprotein: a new type of posttranslational modification of the viral glycoproteins. Cell 17:813–819
    [Google Scholar]
  12. Schnitzer T. J., Lodish H. F. 1979; Noninfectious vesicular stomatitis virus particles deficient in the viral nucleocapsid. Journal of Virology 29:443–447
    [Google Scholar]
  13. Schnitzer T. J., Dickson C., Weiss R. A. 1979; Morphological and biochemical characterization of viral particles produced by tsO45 mutant of vesicular stomatitis virus at restrictive temperature. Journal of Virology 29:185–195
    [Google Scholar]
  14. Simonsen C., Batt-Humphries S., Summers D. F. 1979; RNA synthesis in vesicular stomatitis virus-infected cells: in vivo regulation of replication. Journal of Virology 31:124–132
    [Google Scholar]
  15. Toneguzzo F., Ghosh H. P. 1978; In vitro synthesis of vesicular stomatitis virus membrane glycoprotein and insertion into membranes. Proceedings of the National Academy of Sciences of the United States of America 75:715–719
    [Google Scholar]
  16. Wertz G. W. 1978; Isolation of possible replicative intermediate structures from vesicular stomatitis virus infected cells. Virology 85:271–285
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-60-1-165
Loading
/content/journal/jgv/10.1099/0022-1317-60-1-165
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error