An Invertebrate Calici-like Virus: Evidence for Partial Virion Disintegration in Host Excreta Free

Abstract

SUMMARY

A virus with morphological and physicochemical properties similar to those of the vertebrate caliciviruses was isolated from navel orangeworms, (Walker). Infected larvae contained two types of virus particles: a 185S, 38 nm cupped particle (ACSVi) with a single major polypeptide of 70000 mol. wt. and a 165S, 28 nm smooth particle (ACSVii) with a single major polypeptide of 29000 mol. wt. Larval frass contained a heterogeneous population of virus particles. Evidence is presented which suggests that the 38 nm particle degrades by proteolytic digestion to produce predominantly 28 nm particles in frass. Virus particles contained a single-stranded RNA of 36S (about 2.5 × 10 mol. wt.).

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/content/journal/jgv/10.1099/0022-1317-60-1-115
1982-05-01
2024-03-28
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References

  1. Brakke M. K., Van pelt N. 1970; Linear-log sucrose gradients for estimating sedimentation of plant viruses and nucleic acids. Analytical Biochemistry 38:56–64
    [Google Scholar]
  2. Burroughs J. N., Brown F. 1974; Physico-chemical evidence for reclassification of the caliciviruses. Journal of General Virology 22:281–286
    [Google Scholar]
  3. Burroughs J. N., Doel T. R., Smale C. J., Brown F. 1978; A model for vesicular exanthema virus, the prototype of the calicivirus group. Journal of General Virology 40:161–174
    [Google Scholar]
  4. Curry A., Roberts J. L. 1980; Realistic look at small, round faecal viruses found by electron microscopy. Lancet i:99
    [Google Scholar]
  5. Kellen W. R., Hoffman D. F. 1981; A pathogenic nonoccluded virus in hemocytes of the navel orangeworm, Amyelois transitella. Journal of Invertebrate Pathology 38:52–66
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Longworth J. F. 1978; Small isometric viruses of invertebrates. Advances in Virus Research 23:103–157
    [Google Scholar]
  8. Matthews R. E. F. 1980; Classification and nomenclature of viruses. Intervirology 12:129–296
    [Google Scholar]
  9. Morris T. J., Dodds A. J. 1979; Isolation and analysis of double-stranded RNA from virus infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  10. Morris T. J., Hess R. T., Pinnock D. E. 1979; Physicochemical characterization of a small RNA virus associated with baculovirus infection in Trichoplusia ni. Intervirology 11:238–247
    [Google Scholar]
  11. Reinganum C., O’loughlin G. T., Hogan T. W. 1970; A nonoccluded virus of the field crickets Teleogryllus oceanus and T. commodus (Orthoptera:Gryllidae). Journal of Invertebrate Pathology 16:216–220
    [Google Scholar]
  12. Reinganum C., Robertson J. S., Tinsley T. W. 1978; A new group of RNA viruses from insects. Journal of General Virology 40:195–202
    [Google Scholar]
  13. Schaffer F. L. 1979; Caliciviruses. In Comprehensive Virology vol 14: pp 249–284 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  14. Schaffer F. L., Bachrach H. L., Brown F., Gillespie J. G., Burroughs J. N., Madin S. H., Madeley C. R., Povey R. C., Scott F., Smith A. W., Studdert M. J. 1980; Caliciviridae. Intervirology 14:1–6
    [Google Scholar]
  15. Smith A. W., Skilling D. E., Brown R. J. 1980a; Preliminary investigation of a possible lungworm (Paraphilaroides decorus), fish (Girella nigricans), and marine animal (Callorhinus urcinos) cycle for San Miguel sea lion virus type 5. American Journal of Veterinary Research 41:1845–1849
    [Google Scholar]
  16. Smith A. W., Skilling D. E., Dardiri A. H., Lathan A. B. 1980b; Calicivirus pathogenic for swine: a new serotype isolated from opaleye Girella nigricans, an ocean fish. Science 209:940–941
    [Google Scholar]
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