1887

Abstract

Summary

A particle weight of 8·50 × 10 for encephalomyocarditis virus was derived by combining results from (), sedimentation velocity measurements on highly purified preparations of virus (0·1 to 5 mg./ml.) in 0·1 -KCl + 0·02 -phosphate buffer, pH 8·0, which gave an of 162·3 s, (), differential sedimentation studies of virus in the same solvent but containing various ratios of DO to HO which gave a partial specific volume of 0·678 ml./g., with results from (), analysis of boundary spreading during low speed centrifugation of dilute virus solutions which led to a diffusion coefficient of 1·44 × 10 cm./sec. Sedimentation equilibrium studies on similar virus preparations in the same solvent gave a particle weight of 8·52 × 10. This suggested a hydrated particle diameter for the virus of 29·8 nm. and a frictional ratio of 1·130 which was consistent with that of a hydrated sphere containing 0·29 g. water/g. dry virus.

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1970-03-01
2022-01-23
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