Ribonucleic Acid Content of Encephalomyocarditis Virus Free

Abstract

Summary

The RNA content of encephalomyocarditis virus was found to be 31·7 %, a figure obtained by combining results from nitrogen and phosphorus determinations which gave 32·0 %, from extinction measurements at 260 nm. on perchloric acid hydrolysates which gave 31·3% and from orcinol determinations which gave 31·8%. Specific extinction coefficients obtained were at 260 nm. = 77·4 for the virus in 0·1 m-KCI+ 0·02 -phosphate buffer, pH 8·0, and at 260 nm.= 221 for its RNA in 0·0 -tris + HCl, pH 7·2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-6-3-373
1970-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/6/3/JV0060030373.html?itemId=/content/journal/jgv/10.1099/0022-1317-6-3-373&mimeType=html&fmt=ahah

References

  1. Beaven G. H., Holiday E. R. 1952; Ultraviolet absorption spectra of proteins and amino acids. Advances in Protein Chemistry 7:319
    [Google Scholar]
  2. Beaven G. H., Holiday E. R., Johnson E. A. 1955; Optical properties of nucleic acids and their components. In Nucleic Acids 1493 Chargaff E., Davidson J. N. Ed. by New York: Academic Press;
    [Google Scholar]
  3. Burness A. T. H. 1969; Purification of encephalomyocarditis virus. Journal of General Virology 5:291
    [Google Scholar]
  4. Burness A. T. H., Clothier F. W. 1970; Particle weight and other biophysical properties of encephalo-myocarditis virus. Journal of General Virology 6:381
    [Google Scholar]
  5. Faulkner P., Martin E. M., Sved S., Valentine R. C., Work T. S. 1961; Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 2. The isolation, crystallization and chemical characterization of mouse encephalomyocarditis virus. Biochemical Journal 80:597
    [Google Scholar]
  6. Fenner F. 1968 The Biology of Animal Viruses 1 Molecular and Cellular Biology New York and London: Academic Press;
    [Google Scholar]
  7. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375
    [Google Scholar]
  8. Gierer A., Schramm G. 1956; Infectivity of ribonucleic acid from tobacco mosaic virus. Nature; London: 177702
    [Google Scholar]
  9. Griswold B. c., Humoller F. L., Mcintyre A. R. 1951; Inorganic phosphate and phosphate esters in tissue extracts. Analytical Chemistry 23:192
    [Google Scholar]
  10. Hoskins J. M., Sanders F. K. 1957; Propagation of mouse encephalomyocarditis virus in ascites tumor cells maintained in vitro. British Journal of Experimental Pathology 38:268
    [Google Scholar]
  11. Hurlbert R. B., Schmitz H., Brumm A. F., Potter V. R. 1954; Nucleotide metabolism. II. Chromatographic separation of acid soluble nucleotides. Journal of Biological Chemistry 209:23
    [Google Scholar]
  12. Jacobs S. 1960; The determination of nitrogen in organic compounds by the indanetrione hydrate method. The Analyst 85:257
    [Google Scholar]
  13. Kaighn M. E., Moscarello M. A., Fuerst C. R. 1964; Purification of murine encephalomyocarditis virus. Virology 23:183
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  15. Markham R. 1962; The analytical ultracentrifuge as a tool for the investigation of plant viruses. Advances in Virus Research 9:241
    [Google Scholar]
  16. Markham R., Smith J. D. 1951; Chromatographic studies of nucleic acids. 4. The nucleic acid of the turnip yellow mosaic virus, including a note on the nucleic acid of the tomato bushy stunt virus. Biochemical Journal 49:401
    [Google Scholar]
  17. Rueckert R. R., Schäfer W. 1965; Studies on the structure of viruses of the Columbia SK group, 1. Purification and properties of ME virus grown in Ehrlich ascites cell suspensions. Virology 26:333
    [Google Scholar]
  18. Scraba D. G., Kay C. M., Colter J. S. 1967; Physico-chemical studies of three variants of Mengo virus and their constituent ribonucleates. Journal of Molecular Biology 26:67
    [Google Scholar]
  19. Smith J. D., Markham R. 1950; Chromatographic studies on nucleic acids. 2. The quantitative analysis of ribonucleic acids. Biochemical Journal 46:509
    [Google Scholar]
  20. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentose nucleic acids. Biochemical Journal 48:584
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-6-3-373
Loading
/content/journal/jgv/10.1099/0022-1317-6-3-373
Loading

Data & Media loading...

Most cited Most Cited RSS feed