1887

Abstract

Summary

In HeLa cells infected with poliovirus, shifts from optimal to supraoptimal temperatures in the middle of the infectious cycle resulted in extensive degradation of newly made virus RNA. This temperature-induced degradation was not observed until about 3 hr after infection. It did not require simultaneous synthesis of proteins. During the first 3 hr of infection at 39·5°, RNA of temperature-resistant poliovirus strains replicated much faster than at 36°. Subsequently, degradation became predominant. Thus, temperature-resistant strains partially escaped the effects of degradation by faster replication. During the first 150 min. approximately, the RNA of temperature-sensitive strains replicated as well at supraoptimal as at optimal temperatures. Subsequently, newly made virus RNA was degraded. The inhibitory effect of supraoptimal temperatures may be due to release, or activation, of a ribonuclease.

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1970-02-01
2024-04-19
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References

  1. Amako K., Dales S. 1967; Cytopathology of mengovirus infection: I. Relationship between cellular disintegration and virulence. Virology 33:184
    [Google Scholar]
  2. Bablanian R., Eggers H. J., Tamm I. 1965; Studies on the mechanism of poliovirus induced cell damage: II. The relation between poliovirus growth and virus induced morphological changes in cell. Virology 26:114
    [Google Scholar]
  3. Baltimore D., Girard M., Darnell J. E. 1966; Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology 29:179
    [Google Scholar]
  4. Baltimore D., Eggers H. H., Franklin R. M., Tamm I. 1963; Poliovirus induced RNA polymerase and the effects of virus specific inhibitors on its production. Proceedings of the National Academy of Science of the United States of America 49:843
    [Google Scholar]
  5. Braunwald J., Guir J., Burkard G., Kirn A. 1968; Inhibition de la synthèse de l’ADN du virus vaccinal à température supra-optimale sur cellules KB. Compte rendu hebdomadaire des séances de l’Academie des sciences, Paris 267:673
    [Google Scholar]
  6. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279
    [Google Scholar]
  7. Cooper P. D. 1964; The mutation of poliovirus by 5 fluorouracil. Virology 22:186
    [Google Scholar]
  8. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584
    [Google Scholar]
  9. Ennis H. L., Lubin M. 1964; Cycloheximide : aspects of inhibition of protein synthesis in mammalian cells. Science; New York: 1461474
    [Google Scholar]
  10. Fiszman M. Y., Menard-Bucchini D., Lwoff A. 1968; Intervention d’une nucléase dans faction inhibitrice des températures supra-optimales sur le développement d’un poliovirus. Compte rendu hebdomadaire des séances de l’Academie des sciences, Paris 266:1342
    [Google Scholar]
  11. Flanagan J. F. 1966; Hydrolytic enzymes in KB cells infected with poliovirus and herpes simplex virus. Journal of Bacteriology 91:789
    [Google Scholar]
  12. Girard M., Baltimore D. 1966; The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA. Proceedings of the National Academy of Science of the United States of America 56:999
    [Google Scholar]
  13. Girard M., Baltimore D., Darnell J. E. 1967; The poliovirus replication complex: site for synthesis of poliovirus RNA. Journal of Molecular Biology 24:59
    [Google Scholar]
  14. Lwoff A. 1962; The thermosensitive critical event of the viral cycle. Cold Spring Harbor Symposium on Quantitative Biology 27:159
    [Google Scholar]
  15. Lwoff A., Lwoff M. 1958; L’inhibition du développement du virus poliomyélitique à 39° et le problème du rôle de l’hyperthermie dans l’évolution des infections virales. Compte rendu hebdomadaire des séances de l’Académie des sciences, Paris 246:190
    [Google Scholar]
  16. Lwoff A., Lwoff M. 1960; Sur les facteurs du développement viral et leur rôle dans l’évolution de l’infection. Annals Institut Pasteur 98:173
    [Google Scholar]
  17. Lwoff A., Lwoff M. 1961; Les évènements cycliques du cycle viral. I Effets de la température. Annals Institut Pasteur 101:469
    [Google Scholar]
  18. Lwoff A. 1969; Death and transfiguration of a problem. Bacteriological Review 33:390
    [Google Scholar]
  19. Nichol F. R., Tershak D. R. 1968; Rescue of temperature sensitive poliovirus. Journal of Virology 2:415
    [Google Scholar]
  20. Penman S., Becker Y., Darnell J. E. 1964; A cytoplasmic structure involved in the synthesis and assembly of poliovirus component. Journal of Molecular Biology 8:541
    [Google Scholar]
  21. Priess H., Eggers H. J. 1968; Synthesis and activity of RNA polymerase of a temperature sensitive poliovirus mutant at an elevated temperature. Nature; London: 2201047
    [Google Scholar]
  22. Tershak D. R. 1969; Synthesis of RNA in cells infected with LSc poliovirus at elevated temperatures. Journal of Virology 3:297
    [Google Scholar]
  23. Thacore H., Wolff D. A. 1968; Activation of isolated lysosomes by poliovirus infected cell extract. Nature; London: 2181063
    [Google Scholar]
  24. Wentwcrth B. B., Mccahon D., Cooper P. D. 1968; Production of infectious RNA and serum-blocking antigen by poliovirus temperature-sensitive mutants. Journal of General Virology 2:297
    [Google Scholar]
  25. Willems M., Penman S. 1967; The mechanism of host cell protein synthesis inhibition by poliovirus. Virology 30:355
    [Google Scholar]
  26. Wolff D. A., Bubel H. C. 1964; The disposition of lysosomal enzymes as related to specific viral cytopathic effects. Virology 24:502
    [Google Scholar]
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