%0 Journal Article %A Wood, K. R. %A Gould, E. A. %A Smith, H. %T Purification of Semliki Forest Virus Using Disulphide-linked Immunosorbents Derived from Rabbit or Fowl Antibody %D 1970 %J Journal of General Virology, %V 6 %N 2 %P 257-265 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-6-2-257 %I Microbiology Society, %X SUMMARY Semliki Forest virus in the crude supernatant from a culture of chick embryo cells was specifically adsorbed by a disulphide-linked immunosorbent prepared from the γ-globulins of rabbits immunized by Semliki Forest virus grown in mouse brain. Two to 6 ml. of rabbit antiserum of neutralization index 4 to 5 yielded 10 mg. of immunosorbent which bound 1 to 6 × 1010 p.f.u. from 2 to 30 ml. of culture supernatant. Virus, free from most chick cell constituents, could be eluted from the immunosorbents by treatment with bicarbonate/carbonate buffer at pH ii-i. The immunosorbents could be cycled at least five times and about 50 % of the adsorbed virus infectivity recovered at each cycle after the first, from which only 10 to 20 % was recovered. In capacity experiments, immunosorbents derived from chicken antisera against Semliki Forest virus grown in chick cells showed no advantages over those derived from rabbit antisera. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-6-2-257