1887

Abstract

SUMMARY

The lytic bacteriophage W-14 was isolated from sewage using no. 14 as host. The phage had an icosahedral head some 85 nm. in diameter and a contractile tail some 140 nm. long. W-14 formed plaques on only a few strains of . The phage gave biphasic absorption kinetics, with an adsorption constant of 1·9 × 10 ml./min. The latent period was 60 min. and the burst size was about 300. The burst size was dependent upon culture age. The . for inactivation of W-14 was 4·35 min.. was shown to possess a potent photoreactivating system. The heat of inactivation of W-14 was calculated to be 75,700 cal./mole. The phage gave biphasic thermal inactivation kinetics at 55° and 60° but not at 65°. The phage mutated spontaneously to a different plaque type. This mutation affected the adsorptive properties, the thermal sensitivity and the burst size of the phage.

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1970-01-01
2022-01-28
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