1887

Abstract

SUMMARY

Sixteen mutants were isolated from influenza virus type A, strain , after treatment with either fluorouracil or hydroxylamine. Cells doubly infected with several pairs of mutants yielded a high proportion of wild-type virus, probably because of genetic recombination. Recombination frequencies with particular pairs of mutants were very variable, even in replicate tubes of the same experiment, until infected cells were treated with receptor-destroying enzyme () after adsorption of virus. Day-to-day variation between experiments could not be eliminated, but was allowed for by including a standard cross in all experiments, and relating recombination frequencies to the values obtained in this cross. Standardized recombination frequencies were then reproducible and characteristic of each pair of mutants.

The recombination frequencies thus obtained allowed certain of the mutants to be placed in a sequence with additivity between the intervals, suggesting that influenza virus may have a linear genetic map.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-6-1-63
1970-01-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/6/1/JV0060010063.html?itemId=/content/journal/jgv/10.1099/0022-1317-6-1-63&mimeType=html&fmt=ahah

References

  1. Burnet F. M., Lind P. E. 1949; Recombination of characters between two influenza virus strains. Australian Journal of Science 12:109
    [Google Scholar]
  2. Cairns H. J. F., Mason P. J. 1953; Production of influenza A virus in the cells of the allantois. Journal of Immunology 71:38
    [Google Scholar]
  3. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584
    [Google Scholar]
  4. Duesberg P. H. 1968; The RNA’s of influenza virus. Proceedings of the National Academy of Sciences, U.S.A 59:930
    [Google Scholar]
  5. Fazekas De St. Groth S. 1948; Destruction of influenza virus receptors in the mouse lung by an enzyme from V. cholerae . Australian Journal of Experimental Biology and Medical Science 26:29
    [Google Scholar]
  6. Fazekas De St. Groth S., White D. O. 1958; An improved assay for the infectivity of influenza viruses. Journal of Hygiene, Cambridge 56:151
    [Google Scholar]
  7. Fazekas De St. Groth S., Graham D. M., Jack I. 1958; The serology of mumps infections. I. A new source of antigen and a simplified complement-fixation test. Journal of Laboratory and Clinical Medicine 51:883
    [Google Scholar]
  8. Fenner F. 1969; Conditional lethal mutants of animal viruses. Current Topics in Microbiology 48: in Press
    [Google Scholar]
  9. Hirst G. K. 1962; Genetic recombination with Newcastle disease virus, polioviruses, and influenza. Cold Spring Harbor Symposia on Quantitative Biology 27:303
    [Google Scholar]
  10. Kilbourne E. D. 1963; Influenza virus genetics. Progress in Medical Virology 5:79
    [Google Scholar]
  11. Pons M. W., Hirst G. K. 1968a; Polyacrylamide gel electrophoresis of influenza virus RNA. Virology 34:385
    [Google Scholar]
  12. Pons M. W., Hirst G. K. 1968b; Polyacrylamide gel electrophoresis of the replicative form of influenza virus RNA. Virology 35:182
    [Google Scholar]
  13. Simpson R. W. 1964; Genetic studies with influenza A virus. In Cellular Biology of Myxovirus Infections pp 187–206 Wolstenholme G. E. W., Knight J. Ed. by Ciba Foundation Sym-posium London: Churchill;
    [Google Scholar]
  14. Simpson R. W., Hirst G. K. 1961; Genetic recombination among influenza viruses. I. Cross reactivation of plaque-forming capacity as a method for selecting recombinants from the progeny of crosses between influenza A strains. Virology 15:436
    [Google Scholar]
  15. Simpson R. W., Hirst G. K. 1968; Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology 35:41
    [Google Scholar]
  16. Stone J. D. 1948; Prevention of virus infection with enzyme of V. cholerae. I. Studies with viruses of mumps-influenza group in chick embryos. Australian Journal of Experimental Biology and Medical Science 26:49
    [Google Scholar]
  17. Sugiura A., Kilbourne E. D. 1966; Genetic studies of influenza viruses. III. Production of plaque-type recombinants with A0 and A1 strains. Virology 29:84
    [Google Scholar]
  18. Thiry L. 1963; Chemical mutagenesis of Newcastle disease virus. Virology 19:225
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-6-1-63
Loading
/content/journal/jgv/10.1099/0022-1317-6-1-63
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error