Sixteen mutants were isolated from influenza virus type A, strain , after treatment with either fluorouracil or hydroxylamine. Cells doubly infected with several pairs of mutants yielded a high proportion of wild-type virus, probably because of genetic recombination. Recombination frequencies with particular pairs of mutants were very variable, even in replicate tubes of the same experiment, until infected cells were treated with receptor-destroying enzyme () after adsorption of virus. Day-to-day variation between experiments could not be eliminated, but was allowed for by including a standard cross in all experiments, and relating recombination frequencies to the values obtained in this cross. Standardized recombination frequencies were then reproducible and characteristic of each pair of mutants.

The recombination frequencies thus obtained allowed certain of the mutants to be placed in a sequence with additivity between the intervals, suggesting that influenza virus may have a linear genetic map.


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