Observations on the Structure of the Nucleocapsids of some Paramyxoviruses Free

Abstract

SUMMARY

The intact particles and nucleocapsids of mumps, Sendai and measles viruses are of closely similar appearance, size and structure. The intact particles are about 150 nm. in diameter. The filamentous nucleocapsids have a modal length of about 1·1 m., and are constructed of subunits arranged as a single start helix of pitch 5·0 m. for Sendai virus, and about 6·0 m. for mumps and measles viruses. A (helical) projection of the structure of the Sendai nucleocapsid calculated from an electron micrograph showed that the structural subunits are hour-glass-shaped and are arranged in the helix with their long axes inclined at an angle of about 60° to the long axis of the particle. There are probably 11 or 13 subunits in each turn of the basic helix. Optical diffraction patterns of electron micrographs of mumps and measles nucleocapsids show that they have closely similar structures.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-6-1-141
1970-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/6/1/JV0060010141.html?itemId=/content/journal/jgv/10.1099/0022-1317-6-1-141&mimeType=html&fmt=ahah

References

  1. Choppin P. W., Stoeckenius W. 1964; The morphology of SV 5 virus. Virology 23:195
    [Google Scholar]
  2. Compans R. W., Choppin P. W. 1967a; The length of the nucleocapsid of Newcastle disease virus. Virology 33:344
    [Google Scholar]
  3. Compans R. W., Choppin P. W. 1967b; Isolation and properties of the helical nucleocapsid of parainfluenza SV 5. Proceedings of the National Academy of Sciences of the United States of America 57:949
    [Google Scholar]
  4. Compans R. W., Choppin P. W. 1968; The nucleic acid of parainfluenza virus SV 5. Virology 35:289
    [Google Scholar]
  5. Derosier D. J., Klug A. 1968; Reconstruction of three-dimensional structures from electron micrographs. Nature; London: 217130
    [Google Scholar]
  6. Evans M. J., Kingsbury D. W. 1969; Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis. Virology 37:597
    [Google Scholar]
  7. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  8. Haslam E. A., Cheyne I. M., White D. O. 1969; The structural proteins of Newcastle disease virus. Virology 39:118
    [Google Scholar]
  9. Horne R. W., Waterson A. P. 1960; A helical structure in mumps, Newcastle disease and Sendai viruses. Journal of Molecular Biology 2:75
    [Google Scholar]
  10. Horne R. W., Waterson A. P., Wildy P., Farnham A. E. 1960; The structure and composition of the myxoviruses. I. Electron microscope studies of the structure of myxovirus particles by negative staining techniques. Virology 11:79
    [Google Scholar]
  11. Hosaka Y. 1968; Isolation and structure of the nucleocapsid of HVJ. Virology 35:445
    [Google Scholar]
  12. Hosaka Y., Shimizu K. 1968; Lengths of the nucleocapsids of Newcastle disease and mumps viruses. Journal of Molecular Biology 35:369
    [Google Scholar]
  13. Hosaka Y., Kitano H., Ikeguchi S. 1966; Studies on the pleomorphism of HVJ virions. Virology 29:205
    [Google Scholar]
  14. Hosaka Y., Nishi Y., Fukai K. 1961; The structure of HVJ. II. The fine structure of the subunits. Bikens Journal 2:243
    [Google Scholar]
  15. Hoyle L. 1952; Structure of the influenza virus. The relation between biological activity and the chemical structure of virus fractions. Journal of Hygiene 50:229
    [Google Scholar]
  16. Hoyle L., Horne R. W., Waterson A. P. 1961; The structure and composition of the myxoviruses. II. Components released from the influenza particle by ether. Virology 13:448
    [Google Scholar]
  17. Kingsbury D. W., Darlington R. W. 1968; Isolation and properties of Newcastle disease virus nucleocapsid. Journal of Virology 2:248
    [Google Scholar]
  18. Klenk H. D., Choppin P. W. 1969; Chemical composition of the parainfluenza virus SV 5. Virology 37:155
    [Google Scholar]
  19. Klug A., Berger J. E. 1964; An optical method for the analysis of periodicities in electron micrographs, and some observations on the mechanism of negative staining. Journal of Molecular Biology 10:565
    [Google Scholar]
  20. Klug A., Crick F. H. C., Wyckoff H. W. 1958; Diffraction by helical structures. Acta Crystallographica 11:199
    [Google Scholar]
  21. Schäfer W., Zillig W. 1954; Über den Aufbau des Virus-Elementarteilchens der klassischen Geflügelpest. I. Gewinnung, physikalischchemische und biologische Eigenschaften einiger Spaltprodukte. Zeitschrift für Naturforschung 9b:779–788
    [Google Scholar]
  22. Wrigley N. G. 1968; The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy. Journal of Ultrastructure Research 24:454
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-6-1-141
Loading
/content/journal/jgv/10.1099/0022-1317-6-1-141
Loading

Data & Media loading...

Most cited Most Cited RSS feed