%0 Journal Article %A Kapoor, A. K. %A Ling, N. R. %A Nash, A. A. %A Buchan, A. %A Wildy, P. %T In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens %D 1982 %J Journal of General Virology, %V 59 %N 2 %P 415-419 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-59-2-415 %K rabbit %K T cell stimulation %K infected cells %K HSV %I Microbiology Society, %X SUMMARY Lymphocyte stimulation responses to herpes antigens were studied using virus-infected X-irradiated cells. Rabbits were immunized with herpes simplex virus type 1 (strain HFEM) grown in RK13 cells. For in vitro stimulation assay BHK21 cells were X-irradiated (15000 rad) and infected with a high m.o.i. of a temperature-sensitive (ts) mutant (N102) of HFEM strain at the non-permissive temperature (38.5 °C) of virus. Virus antigens were expressed on the infected cells and there was no leakage of infectious virus into the medium at 38.5 °C. T lymphocytes from rabbits immunized with herpes simplex virus were specifically activated by herpesvirus-infected X-irradiated cells; lymph node cells from rabbits immunized with RK13 cells and from non-immune rabbits showed no proliferative response. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-59-2-415