1887

Abstract

SUMMARY

Some structural properties of the vesicular stomatitis virus (VSV) G protein were examined in virions and isolated envelope fragments. We have shown that in the virion a portion of the G protein extends through the lipid envelope and that this part of the molecule can be cleaved by chymotrypsin. Envelope fragments isolated from VSV without the use of detergents maintained the structural characteristics of the G protein found in intact virions. In addition, we provide evidence that at least some of the isolated envelope fragments have both sides of the bilayer exposed to added reagents, suggesting that this preparation would be useful for reassociation experiments.

Keyword(s): envelope , G protein , structure and VSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-59-2-319
1982-04-01
2022-08-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/59/2/JV0590020319.html?itemId=/content/journal/jgv/10.1099/0022-1317-59-2-319&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Brown F., Smale C. J., Horzinek M. C. 1974; Lipid and protein organization in vesicular stomatitis and Sindbis viruses. Journal of General Virology 22:455–458
    [Google Scholar]
  3. Carroll A. R., Wagner R. R. 1979; Role of the membrane (M) protein in endogenous inhibition of in vitro transcription by vesicular stomatitis virus. Journal of Virology 29:134–142
    [Google Scholar]
  4. Eger R., Compans R. W., Rifkin D. B. 1975; The organization of the proteins of vesicular stomatitis virions: labeling with pyridoxal phosphate. Virology 66:610–615
    [Google Scholar]
  5. Emerson S. U., Wagner R. R. 1972; Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions. Journal of Virology 10:297–309
    [Google Scholar]
  6. Hartree E. F. 1972; Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48:422–427
    [Google Scholar]
  7. Irving R. A., Toneguzzo F., Rhee S. H., Hofmann T., Ghosh H. P. 1979; Synthesis and assembly of membrane glycoproteins: presence of leader peptide in nonglycosylated precursor of membrane glycoprotein of vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 76:570–574
    [Google Scholar]
  8. Katz F. N., Rothman J. E., Lingappa V., Blobel G., Lodish H. F. 1977; Membrane assembly in vitro: synthesis, glycosylation and asymmetric insertion of a transmembrane protein. Proceedings of the National Academy of Sciences of the United States of America 74:3278–3282
    [Google Scholar]
  9. Knife D., Baltimore D., Lodish H. F. 1977a; Maturation of viral proteins in cells infected with temperature-sensitive mutants of vesicular stomatitis virus. Journal of Virology 21:1149–1158
    [Google Scholar]
  10. Knipe D., Lodish H. F., Baltimore D. 1977b; Analysis of the defects of temperature-sensitive mutants of vesicular stomatitis virus: intracellular degradation of specific viral proteins. Journal of Virology 21:1140–1148
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lingappa V. R., Katz F. N., Lodish H. F., Blobel G. 1978; A signal sequence for the insertion of a transmembrane glycoprotein. Journal of Biological Chemistry 253:8667–8670
    [Google Scholar]
  13. Lodish H. F., Porter M. 1980a; Heterogeneity of vesicular stomatitis virus particles: implications for virion assembly. Journal of Virology 33:52–58
    [Google Scholar]
  14. Lodish H. F., Porter M. 1980b; Specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles. Cell 19:161–169
    [Google Scholar]
  15. Lodish H. F., Weiss R. A. 1979; Selective isolation of mutants of vesicular stomatitis virus defective in production of the viral glycoprotein. Journal of Virology 30:177–189
    [Google Scholar]
  16. McSharry J. J., Compans R. W., Choppin P. W. 1971; Proteins of vesicular stomatitis virus and of phenotypically mixed vesicular stomatitis virus-simian virus 5 virions. Journal of Virology 8:722–729
    [Google Scholar]
  17. McSharry J. J., Compans R. W., Lackland H., Choppin P. W. 1975; Isolation and characterization of the nonglycosylated membrane protein and a nucleocapsid complex from the paramyxovirus SV5. Virology 67:365–374
    [Google Scholar]
  18. Moore N. F., Kelley J. M., Wagner R. R. 1974; Envelope proteins of vesicular stomatitis virions. Accessibility to iodination. Virology 61:292–296
    [Google Scholar]
  19. Morrison T. G., Lodish H. F. 1975; Site of synthesis of membrane and non-membrane proteins of vesicular stomatitis virus. Journal of Biological Chemistry 250:6955–6962
    [Google Scholar]
  20. Mudd J. A. 1974; Glycoprotein fragment associated with vesicular stomatitis virus after proteolytic digestion. Virology 62:573–577
    [Google Scholar]
  21. Neville D. M. Jr 1971; Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. Journal of Biological Chemistry 246:6328–6334
    [Google Scholar]
  22. Schloemer R. H., Wagner R. R. 1975; Association of vesicular stomatitis virus glycoprotein with virion membrane: characterization of the lipophilic tail fragment. Journal of Virology 16:237–249
    [Google Scholar]
  23. Schnitzer T. J., Dickson C., Weiss R. A. 1979; Morphological and biochemical characterization of viral particles produced by the ts 045 mutant of vesicular stomatitis virus at restrictive temperature. Journal of Virology 29:185–195
    [Google Scholar]
  24. Shimizu K., Nakao I. 1975; The smallest protein of Sendai virus: its candidate function of binding nucleocapsid to envelope. Virology 67:427–437
    [Google Scholar]
  25. Taube S. E., Rothfield L. I. 1978; Isolation of the envelope of vesicular stomatitis virus. Journal of Virology 26:730–735
    [Google Scholar]
  26. Toneguzzo F., Ghosh H. P. 1978; In vitro synthesis of vesicular stomatitis virus membrane glycoprotein and insertion into membranes. Proceedings of the National Academy of Sciences of the United States of America 75:715–719
    [Google Scholar]
  27. Wagner R. R. 1975; Reproduction of rhabdoviruses. In Comprehensive Virology vol 4: pp 1–93 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  28. Witte O. N., Baltimore D. 1977; Mechanism of formation of pseudotypes between vesicular stomatitis virus and murine leukemia virus. Cell 11:505–511
    [Google Scholar]
  29. Zavada J. 1976; Viral pseudotypes and phenotypic mixing. Short review. Archives of Virology 50:1–15
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-59-2-319
Loading
/content/journal/jgv/10.1099/0022-1317-59-2-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error