1887

Abstract

SUMMARY

Bovine leukaemia virus (BLV) was found to agglutinate mouse erythrocytes. Under optimal conditions, including the use of neuraminidase-treated erythrocytes, 200 µg/ml of BLV purified from the supernatant fluid of BLV-infected bat cells had haemagglutinating titres of about 512 units. BLV haemagglutination was drastically affected by pH and temperature; maximum agglutination occurred at pH 6 and 4 °C. That the BLV haemagglutinin is a glycoprotein was suggested by the fact that trypsin, potassium periodate or neuraminidase, but not lipid solvents or phospholipase C, significantly reduced the haemagglutinating (HA) activity of purified BLV. Furthermore, purified BLV glycoprotein of mol. wt. 51000 (gp51) had HA activity. The receptors for BLV on mouse erythrocytes were inactivated by proteolytic enzymes but not by sodium deoxycholate or potassium periodate. Neuraminidase treatment of erythrocytes increased their agglutinability fourfold. Haemagglutination is a relatively sensitive test for detecting BLV glycoprotein because 0.4 µg/ml of glycoprotein can be detected by this method. The pH and temperature sensitivity of the BLV HA reaction and specificity for mouse erythrocytes distinguish BLV from that of equine infectious anaemia virus and murine leukaemia virus, the other C type retroviruses known to have HA activity.

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/content/journal/jgv/10.1099/0022-1317-59-1-83
1982-03-01
2024-03-28
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References

  1. Devare S. G., Stephenson J. R. 1977; Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. Journal of Virology 23:443–447
    [Google Scholar]
  2. Diglio C. A., Ferrer J. F. 1976; Induction of syncytia by the bovine C-type leukemia virus. Cancer Research 36:1056–1067
    [Google Scholar]
  3. Ferrer J. F. 1972; Antigenic comparison of bovine type C virus with murine and feline leukemia virus. Cancer Research 32:1871–1877
    [Google Scholar]
  4. Ferrer J. F. 1980; Bovine lymphosarcoma. Advances in Veterinary Science & Comparative Medicine 24:1–66
    [Google Scholar]
  5. Gilden R. V., Long C. W., Hanson M., Toni R., Charman H. P., Oroszlan S. 1975; Characteristics of the major internal protein and RNAdependent DNA polymerase of bovine leukaemia virus. Journal of General Virology 29:305–314
    [Google Scholar]
  6. Graves D. C., Ferrer J. F. 1976; In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Research 36:4152–4159
    [Google Scholar]
  7. Gupta P., Ferrer J. F. 1978; Detection of a precursor of bovine leukemia virus structural protein in purified virions. Annales de Recherches Veterinaires 9:619–626
    [Google Scholar]
  8. Gupta P., Ferrer J. F. 1981; Diagnosis of BLV infection in cattle; a critical evaluation of various serological and direct methods. International Journal of Cancer 28:179–184
    [Google Scholar]
  9. Howe C., Lee T. 1972; Virus-erythrocyte interactions. Advances in Virus Research 17:1–50
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. McDonald H. C., Ferrer J. F. 1976; Detection, quantitation and characterization of the major internal antigen of the bovine leukemia virus by radioimmunoassay. Journal of the National Cancer Institute 57:875–882
    [Google Scholar]
  12. McDonald H. C., Graves D. C., Ferrer J. F. 1976; Isolation and characterization of an antigen of the bovine C-type virus. Cancer Research 36:1251–1257
    [Google Scholar]
  13. Onuma M., Olson C., Driscoll D. M. 1976; Properties of two isolated antigens associated with bovine leukemia virus infection. Journal of the National Cancer Institute 57:571–578
    [Google Scholar]
  14. Schäfer W., Szanto J. 1969; Studies on mouse leukemia virus. II. Nachweis eines virus spezifischen hamagglutinins. Zeitschrift für Natursforschung B24:1324–1331
    [Google Scholar]
  15. Sentsui H., Kono Y. 1976; Hemagglutination by equine infectious anemia virus. Infection and Immunity 14:325–331
    [Google Scholar]
  16. Sentsui H., Kono Y. 1981; Hemagglutination by several strains of equine infectious anemia virus. Archives of Virology 67:75–84
    [Google Scholar]
  17. Smith K. C., Hanawalt P. C. 1969 Molecular Photobiology Inactivation and Recovery pp 86–95 New York & London: Academic Press;
    [Google Scholar]
  18. Weiland F., Ueberschar S. 1976; Ultrastructural comparison of bovine leukemia virus (BLV) with C-type particles of other species. Archives of Virology 52:187–190
    [Google Scholar]
  19. Witter R., Frank H., Moennig V., Hunsmann G., Lange J., Schafer W. 1973; Properties of mouse leukemia virus. IV. Hemagglutination assay and characterization of hemagglutinating surface components. Virology 54:330–345
    [Google Scholar]
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