@article{mbs:/content/journal/jgv/10.1099/0022-1317-59-1-163, author = "Mayo, M. A. and Barker, H. and Robinson, D. J. and Tamada, T. and Harrison, B. D.", title = "Evidence that Potato Leafroll Virus RNA is Positive-stranded, is Linked to a Small Protein and Does Not Contain Polyadenylate", journal= "Journal of General Virology", year = "1982", volume = "59", number = "1", pages = "163-167", doi = "https://doi.org/10.1099/0022-1317-59-1-163", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-59-1-163", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "genome-linked protein", keywords = "potato leafroll virus", keywords = "RNA", keywords = "polyadenylate", abstract = "SUMMARY RNA extracted from particles of potato leafroll virus (PLRV) infected tobacco mesophyll protoplasts. Treating the RNA with proteinase K did not abolish its infectivity. In messenger-dependent rabbit reticulocyte lysate, PLRV RNA induced the synthesis of specific polypeptides: a major product of mol. wt. 71000 but no product the size of coat protein. PLRV RNA is therefore positive-stranded. A genome-linked protein (apparent mol. wt. 7000) was detected in preparations of PLRV RNA but no polyadenylate sequence was found. These features may prove to be characteristic of luteoviruses.", }