To measure the length of heating required to convert a λ lysogen in the immune (im) phase into the anti-immune (im) phase, gene activity was used as an indicator. It was observed that 5 min heating at 41 °C did not shift any lysogenic cells of 594(λ I857 ) from the im phase into the im phase, and it took 17 min heating at 41 °C followed by long hours of culture at 30 °C to shift half of the lysogenic cells into the im phase. Such a length of heating is too long to be accounted for by blocking of the expression of lambda repressor by gene product. The result is more consistent with accumulation of a certain level of gene product during heating so that the synthesis of the repressor is blocked even after a return to low temperature.

Keyword(s): cro gene , immunity , phage and regulation

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