1887

Abstract

SUMMARY

We have constructed a hybrid plasmid molecule that contains the complete coding sequences from the 26S mRNA of Semliki Forest virus. Five fragments which together covered the mRNA sequence were isolated from three original hybrid plasmids and joined together at five different restriction endonuclease cleavage sites using T4 DNA ligase.

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/content/journal/jgv/10.1099/0022-1317-58-2-443
1982-02-01
2022-01-17
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References

  1. Garoff H., Simon K. 1980; The Semliki Forest virus envelope: a probe for studies of plasma membrane structure and assembly. In Biological Chemistry of Organelle Formation pp 221–234 Edited by Bucher T., Sebald W., Weiss H. Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  2. Garoff H., Frischauf A. M., Simons K., Lehrach H., Delius H. 1980a; The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region. Proceedings of the National Academy of Sciences of the United States of America 77:6376–6380
    [Google Scholar]
  3. Garoff H., Frischauf A.M., Simons K., Lehrach H., Delius H. 1980b; Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins. Nature, London 288:236–241
    [Google Scholar]
  4. Guarente L., Lauer G., Roberts T. M., Ptashne M. 1980; Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin. Cell 20:543–553
    [Google Scholar]
  5. Kääriäinen L., Renkonen O. 1977; Envelopes of lipid-containing viruses as models for membrane assembly. In The Synthesis, Assembly and Turnover of Cell Surface Components pp 741–801 Edited by Poste G., Nicolson G. L. Amsterdam: North-Holland;
    [Google Scholar]
  6. Mulligan R. C., Berg P. 1980; Expression of a bacterial gene in mammalian cells. Science 209:1422–1427
    [Google Scholar]
  7. Villa-Komaroff L., Efstratiadis A., Broome S., Lomedico P., Tizard R., Naber S. P., Chick W. L., Gilbert W. 1978; A bacterial clone synthesizing proinsulin. Proceedings of the National Academy of Sciences of the United States of America 75:3727–3731
    [Google Scholar]
  8. Wieslander L. 1979; A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Analytical Biochemistry 98:305–309
    [Google Scholar]
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