1887

Abstract

SUMMARY

Facets of virulence for ferrets of 16 recombinant clones of two parent viruses A/Finland/4/74 (H3N2) and A/Okuda/57 (H2N2) were determined and viewed in relation to their genetic composition. Of the five pairs of recombinant clones with RNA segments of the same parental origin, differences in facets of virulence were detected between members of two of the pairs. One pair differed in ability to produce fever, and another pair in ability to infect the lower respiratory tract. Subsequent analyses indicated slight differences in two genes of the first pair of viruses. Of all the recombinant clones examined the three that derived their surface antigens from the attenuated parent (A/Okuda) were less virulent than the 13 which possessed the surface antigens of the virulent parent (A/Finland). The virulence of the latter clones tended to decrease as the number of RNA segments that were derived from A/Okuda increased.

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1982-02-01
2024-04-25
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References

  1. Arora D. J. S., Hill-Schubert J. 1980; The complete neuraminidase of influenza A/PR/8/34 (H0N1) is not detectable in its recombinant virus. Canadian Journal of Microbiology 26:1369–1373
    [Google Scholar]
  2. Bean W. J., Webster R. G. 1978; Phenotypic properties associated with influenza genome segments. In Negative Strand Viruses and the Host Cell pp 685–692 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  3. Beare A. S. 1978; Recombinant influenza viruses from parents of known virulence for man. In Negative Strand Viruses and the Host Cell pp 745–755 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  4. Bosch F. X., Orlich M., Klenk H.D., Rott R. 1979; The structure of the haemagglutinin, a determinant for the pathogenicity of influenza viruses. Virology 95:197–207
    [Google Scholar]
  5. Brand C., Palese P. 1980; Sequential passage of influenza virus in embryonated eggs or tissue culture: emergence of mutants. Virology 107:424–433
    [Google Scholar]
  6. Campbell D. 1979; Studies on the ferret-virulence of recombinant influenza viruses. Ph,. D. thesis University of Birmingham, U,. K
    [Google Scholar]
  7. Campbell D., Sweet C., Smith H. 1979; Comparisons of virulence of influenza virus recombinants in ferrets in relation to their behaviour in man and their genetic constitution. Journal of General Virology 44:37–44
    [Google Scholar]
  8. Davenport F. M., Hennessy A. V., Massabb H. F., Minuse E., Clark L. C., Abrams G. D., Mitchell J. R. 1977; Pilot studies on recombinants cold-adapted live type A and B influenza virus vaccines. Journal of Infectious Diseases 136:17–25
    [Google Scholar]
  9. Erickson A. H., Kilbourne E. D. 1980; Mutation in the haemagglutinin of A/N-WS/33 influenza virus recombinants influencing sensitivity of trypsin and antigenic reactivity. Virology 107:320–330
    [Google Scholar]
  10. Fenton R. J., Jennings R., Potter C. W. 1977; Differential response of ferrets to infection with virulent and avirulent influenza viruses: a possible marker of virus attenuation. Archives of Virology 55:55–66
    [Google Scholar]
  11. Ghendon Y., Klimov A., Blagoveshenskaya O., Genkina D. 1979; Investigation of recombinants of human influenza and fowl plague viruses. Journal of General Virology 43:183–191
    [Google Scholar]
  12. Hay A. J., Bellamy A. R., Abraham G., Skehel J. J., Brand C. M., Webster R. G. 1977; Procedures for characterization of the genetic material of candidate vaccine strains. In International Symposium of Influenza Immunization vol. II, Geneva 1977. Developments in Biological Standardization 39:15–24 Basel: S Karger;
    [Google Scholar]
  13. Hay A. J., Skehel J. J., Webster R. G. 1979; Differentiation of the haemagglutinin genes of variant influenza viruses by RNA-RNA hybridization. Journal of General Virology 45:245–248
    [Google Scholar]
  14. Kilbourne E. D. 1969; Future influenza vaccines and the use of genetic recombinants. Bulletin of the World Health Organization 41:643–645
    [Google Scholar]
  15. Kilbourne E. D., McGregor S., Easterday B. C. 1979; Haemagglutinin mutants of swine influenza virus differing in replication characteristic in their natural host. Infection and Immunity 26:197–201
    [Google Scholar]
  16. Koprowski H., Gerhard W., Croce C. M. 1977; Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proceedings of the National Academy of Sciences of the United States of America 74:2985–2988
    [Google Scholar]
  17. McCahon D., Schild G. C. 1972; Segregation of antigenic and biological characteristics during influenza virus recombination. Journal of General Virology 15:73–77
    [Google Scholar]
  18. McCahon D., Beare A. S., Stealey V. M. 1976; The production of live attenuated influenza A strains by recombination with A/Okuda/57. Postgraduate Medical Journal 52:389–394
    [Google Scholar]
  19. Matsuyama T., Sweet C., Collie M. H., Smith H. 1980; Different aspects of virulence in ferrets exhibited by influenza virus recombinants of known genetic constitution. Journal of Infectious Diseases 141:351–361
    [Google Scholar]
  20. Mayer V., Schulman J. L., Kilbourne E. D. 1973; Nonlinkage of neurovirulence exclusively to viral haemagglutinin or neuraminidase in genetic recombinants of A/NWS (H0N1) influenza virus. Journal of Virology 11:272–278
    [Google Scholar]
  21. Oxford J. S., McGeoch D. J., Schild G. C., Beare A. S. 1978; Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature, London 273:778–779
    [Google Scholar]
  22. Rott R. 1979; Molecular basis of infectivity and pathogenicity of myxovirus. Archives of Virology 59:285–298
    [Google Scholar]
  23. Rott R., Orlich M., Scholtissek C. 1976; Attenuation of pathogenicity of fowl plague virus by recombination with other influenza A viruses non-pathogenic for fowl: non exclusive dependence of pathogenicity on haemagglutinin and neuraminidase of the virus. Journal of Virology 19:54–60
    [Google Scholar]
  24. Scholtissek C., Vallbracht A., Flehmig B., Rott R. 1979; Correlation of pathogenicity and gene constellation of influenza A viruses. II. Highly neurovirulent recombinants derived from non-neurovirulent or weakly neurovirulent parent virus strains. Virology 95:492–500
    [Google Scholar]
  25. Schulman J. L., Palese P. 1977; Virulence factors of influenza A viruses: WSN virus neuraminidase required for plaque production in MDBK cells. Journal of Virology 24:170–176
    [Google Scholar]
  26. Sweet C., Smith H. 1980; Pathogenicity of influenza virus. Microbiological Reviews 44:303–330
    [Google Scholar]
  27. Sweet C., Stephen J., Smith H. 1974; Purification of influenza viruses using disulphide-linked immunosorbents derived from rabbit antibody. Immunochemistry 11:295–304
    [Google Scholar]
  28. Thompson W. R. 1947; Use of moving averages and interpolation to estimate median-effective dose. I. Fundamental formulas, estimation of error, and relation to other methods. Bacteriological Reviews 11:115–145
    [Google Scholar]
  29. Toms G. L., Bird R. A., Kingsman S. M., Sweet C., Smith H. 1976; The behaviour in ferrets of two closely related clones of influenza virus of differing virulence for man. British Journal of Experimental Pathology 57:37–48
    [Google Scholar]
  30. Toms G. L., Davies J. A., Woodward C. G., Sweet C., Smith H. 1977; The relation of pyrexia and nasal inflammatory response to virus levels in nasal washings of ferrets infected with influenza viruses of differing virulence. British Journal of Experimental Pathology 58:444–458
    [Google Scholar]
  31. Vallbracht A., Flehmig B., Gerth H.J. 1979; Influenza virus: appearance of high mouse-neurovirulent recombinants. Intervirology 11:16–22
    [Google Scholar]
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