Persistent Infection with Infectious Pancreatic Necrosis Virus Mediated by Defective-interfering (DI) Virus Particles in a Cell Line Showing Strong Interference but Little DI Replication
The characteristics of chinook salmon embryo cells persistently infected with infectious pancreatic necrosis virus were consistent with defective-interfering (DI) particle-mediated persistence. All the cells were infected and were slowly releasing virus, but they could be cured of virus in the presence of antiserum. Immunofluorescence showed that the amount of virus antigen in persistently infected cells was low. This fact, coupled with the observation that few DI particles were released by these cells, indicated that DI particles were not replicated to excess in this cell line.
ChangN.,
MacdonaldR. D.,
YamamotoT.1978; Morphology and polypeptide composition of ten isolates of infectious pancreatic necrosis virus. Canadian Journal of Microbiology 24:19–27
HedrickR. P.,
FryerJ. L.1981; Persistent infection of three salmonid cell lines with infectious pancreatic necrosis virus (IPNV). Fish Pathology 15:163–172
HedrickR. P.,
LeongJ. C.,
FryerJ. L.1978; Persistent infection in salmonid fish cells with infectious pancreatic necrosis virus (IPNV). Journal of Fish Diseases 1:297–308
HollandJ. J.,
VillarrealL. P.,
BreindlM.1976; Factors involved in the generation and replication of rhabdovirus defective T particles. Journal of Virology 17:805–815
HuangA. S.,
MandersE. K.1972; Ribonucleic acid synthesis in vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering virus particles. Journal of Virology 9:909–916
MacdonaldR. D.1980; Immunofluorescent detection of double stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus. Canadian Journal of Microbiology 26:256–261
MacdonaldR. D.,
DobosP.1981; Identification of the proteins encoded by each genome segment of infectious pancreatic necrosis virus. Virology 114:414–422
MacdonaldR. D.,
YamamotoT.1978; Quantitative analysis of defective interfering particles in infectious pancreatic necrosis virus preparations. Archives of Virology 57:77–89
PerraultJ.,
HollandJ. J.1972; Absence of transcriptase activity or transcription-inhibiting ability in defective interfering particles of vesicular stomatitis virus. Virology 50:159–170
PotterK. N.,
StewartR. B.1976; Comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and L cells. Canadian Journal of Microbiology 22:1458–1463
ScherrerR.,
CohenJ.1975; Studies on infectious pancreatic necrosis virus: interactions with rainbow trout gonad and fathead minnow cells; selection of a variant virus type in fathead minnow cells. Journal of General Virology 28:9–20
StampferM.,
BaltimoreD.,
HuangA. S.1969; Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. Journal of Virology 4:154–161
StarkC.,
KennedyS. I. T.1978; The generation and propagation of defective-interfering particles of Semliki Forest virus in different cell types. Virology 89:285–299
Persistent Infection with Infectious Pancreatic Necrosis Virus Mediated by Defective-interfering (DI) Virus Particles in a Cell Line Showing Strong Interference but Little DI Replication