1887

Abstract

SUMMARY

Infectivity of nucleic acid from highly infectious mouse scrapie brains was studied by transfecting the nucleic acid prior to inoculation into animals. Foetal mouse brain and whole mouse embryo cultures were chosen as the cells used for the transfection. As an internal control, infectious nucleic acid was recovered from bacteriophage φX174 and whole virus particles were obtained when cultures were transfected with herpes simplex virus DNA. In contrast, none of the animals inoculated with nucleic acid preparations derived from scrapie-infected tissues developed scrapie disease either with or without transfection procedures. These findings (i) show transfection techniques fail to elicit evidence of a scrapie-specific infectious nucleic acid and (ii) confirm the observation that scrapie is not a viroid.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-58-2-263
1982-02-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/58/2/JV0580020263.html?itemId=/content/journal/jgv/10.1099/0022-1317-58-2-263&mimeType=html&fmt=ahah

References

  1. Alper T., Haig D. A., Clarke M. C. 1966; The exceptionally small size of the scrapie agent. Biochemical and Biophysical Research Communications 22:278–284
    [Google Scholar]
  2. Alper T., Cramp W. A., Haig D. A., Clarke M. C. 1967; Does the agent of scrapie replicate without nucleic acid?. Nature, London 214:764–767
    [Google Scholar]
  3. Alper T., Haig D. A., Clarke M. C. 1978; The scrapie agent: evidence against its dependence for replication on intrinsic nucleic acid. Journal of General Virology 41:503–516
    [Google Scholar]
  4. Blair D. G., Clewell D. B., Sheratt D. J., Helinski D. R. 1971; Strand-specific supercoiled DNA-protein relaxation complexes of the bacterial plasmids Col El and Col E2. Proceedings of the National Academy of Sciences of the United States of America 68:210–214
    [Google Scholar]
  5. Clarke M. C. 1979; Infection of cell cultures with scrapie agent. In Slow Transmissible Diseases of the Nervous System vol 2: pp 225–242 Edited by Prusiner S. B., Hadlow W. J. New York: Academic Press;
    [Google Scholar]
  6. Diener T. O. 1972; Is the scrapie agent a viroid?. Nature New Biology 235:218–219
    [Google Scholar]
  7. Faust C. H. Jr, Diggelmann H., Macn B. 1973; Isolation of poly(adenylic acid)-rich ribonucleic acid from mouse myeloma and synthesis of complementary deoxyribonucleic acid. Biochemistry 12:925–931
    [Google Scholar]
  8. Field E. J. 1966; Transmission experiments with multiple sclerosis: an interim report. British Medical Journal 2:564–565
    [Google Scholar]
  9. Gibbons R. A., Hunter G. D. 1967; Nature of the scrapie agent. Nature, London 215:1041–1043
    [Google Scholar]
  10. Gibbs C. J. Jr 1967; Search for infectious etiology in chronic and subacute degenerative diseases of the central nervous system. Current Topics in Microbiology and Immunology 40:44–48
    [Google Scholar]
  11. Gibbs C. J. Jr, Gajdusek D. C., Muel B., Latarjet R. 1978; Unusual resistance to ionizing radiation of the viruses of kuru, Creutzfeldt-Jakob disease, and scrapie. Proceedings of the National Academy of Sciences of the United States of America 75:6268–6270
    [Google Scholar]
  12. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5-DNA. Virology 52:456–467
    [Google Scholar]
  13. Graham F. L., Veldhuisen G., Wilkie N. M. 1973; Infectious herpesvirus DNA. Nature New Biology 245:265–266
    [Google Scholar]
  14. Haig D. A., Clarke M. C. 1965; Observations on the scrapie agent. In Slow, Latent and Temperate Virus Infections pp 215–219 Washington D.C.: U.S. Government Printing Office;
    [Google Scholar]
  15. Hansen J. N. 1974; Isolation of higher molecular weight DNA from Bacillus cereus T using proteinase K. Preparative Biochemistry 4:473–488
    [Google Scholar]
  16. Hunter G. D., Millson G. C. 1964; Studies on the heat stability and chromatographic behaviour of the scrapie agent. Journal of General Microbiology 37:251–258
    [Google Scholar]
  17. Hunter G. D., Collis S. C., Millson G. C., Kimberlin R. H. 1976; Search for scrapie-specific RNA and attempts to detect an infectious DNA or RNA. Journal of General Virology 32:157–162
    [Google Scholar]
  18. Kieff E. D., Bachenheimer S. L., Roizman B. 1971; Size, composition and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. Journal of Virology 8:125–132
    [Google Scholar]
  19. Kingsbury D. T., Smeltzer D. A., Gibbs C. J., Gajdusek D. C. 1981; Evidence for normal cell-mediated immunity in scrapie-infected mice. Infection and Immunity 32:1176–1180
    [Google Scholar]
  20. Latarjet R. 1979; Inactivation of the agents of scrapie, Creutzfeldt-Jakob disease, and kuru by radiations. In Slow Transmissible Diseases of the Nervous System vol 2: pp 387–407 Edited by Prusiner S. B., Hadlow W. J. New York: Academic Press;
    [Google Scholar]
  21. Lee Y. F., Nomoto A., Detjen B. H., Wimmer E. 1977; A protein covalently linked to poliovirus genome RNA. Proceedings of the National Academy of Sciences of the United States of America 74:59–63
    [Google Scholar]
  22. Malone T. G., Marsh R. F., Hanson R. P., Semancik J. S. 1979; Evidence for the low molecular weight of scrapie agent. Nature, London 278:575–576
    [Google Scholar]
  23. Marsh R. F., Semancik J. S., Medappa K. C., Hanson R. F., Rueckert R. R. 1974; Scrapie and transmissible mink encephalopathy: search for infectious nucleic acid. Journal of Virology 13:993–996
    [Google Scholar]
  24. Millson G. C. 1965; Lysosomal enzymes in normal and scrapie mouse brain. Journal of Neurochemistry 12:461–468
    [Google Scholar]
  25. Mould D. L., Dawson A. M. 1970; The response in mice to heat-treated scrapie agent. Journal of Comparative Pathology 80:595–600
    [Google Scholar]
  26. Porter D. D., Porter H. G., Cox N. A. 1973; Failure to demonstrate a humoral immune response to scrapie infection in mice. Journal of Immunology 3:1407–1410
    [Google Scholar]
  27. Prusiner S. B., Groth D. F., Cochran S. P., Marsiaz F. R., Mckinley M. P., Martinez H. M. 1980a; Molecular properties, partial purification and assay by incubation period measurements of the hamster scrapie agent. Biochemistry 19:4883–4891
    [Google Scholar]
  28. Prusiner S. B., Groth D. F., Cochran S. P., McKinley M. P., Marsiarz F. R. 1980b; Gel electrophoresis and glass permeation chromatography of the hamster scrapie agent after enzymatic digestion and detergent extraction. Biochemistry 19:4892–4898
    [Google Scholar]
  29. Ransom B. R., Neale E., Henkart M., Bullock P. N., Nelson P. G. 1977; Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties. Journal of Neurophysiology 40:1132–1150
    [Google Scholar]
  30. Sheldrick P., Laithier M., Lando D., Ryhiner M. L. 1973; Infectious DNA from herpes simplex virus: infectivity of double-stranded and single-stranded molecules. Proceedings of the National Academy of Sciences of the United States of America 70:3621–3625
    [Google Scholar]
  31. Stamp J. T., Brotherton J. G., Zlotnik L., Mackay J. M. K., Smith W. 1959; Further studies in scrapie. Journal of Comparative Pathology 69:268–280
    [Google Scholar]
  32. Stow N. D., Wilkie N. M. 1976; An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. Journal of General Virology 33:447–458
    [Google Scholar]
  33. Ward R. L., Porter D. D., Stevens J. G. 1974; Nature of the scrapie agent: evidence against a viroid. Journal of Virology 14:1099–1103
    [Google Scholar]
  34. Zasloff M., Ginder G. D., Felsenfeld G. 1978; A new method for the purification of covalently closed circular DNA molecules. Nucleic Acids Research 5:1139–1152
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-58-2-263
Loading
/content/journal/jgv/10.1099/0022-1317-58-2-263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error