%0 Journal Article %A Paraskeva, Christos %A Brown, Keith W. %A Gallimore, Phillip H. %T Adenovirus–Cell Interactions Early After Infection: In vitro Characteristics and Tumourigenicity of Adenovirus Type 2-transformed Rat Liver Epithelial Cells %D 1982 %J Journal of General Virology, %V 58 %N 1 %P 73-81 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-58-1-73 %K Ad-2 %K Ad-12 %K tumourigenicity %K virus–cell interactions %I Microbiology Society, %X SUMMARY Cloned rat liver epithelial cells (clone C3) were semi-permissive for adenovirus type 2 (Ad-2) and non-permissive for adenovirus type 12 (Ad-12). Ad-2-infected C3 cells were shown to produce hexon and fibre protein, but at an m.o.i. of 20 a maximum virus yield of only 2.4 p.f.u. per cell was obtained. Forty-eight h after infection with Ad-12 ‘early’ virus proteins (major species 8K and 60K), but no ‘late’ proteins (virus structural proteins) could be identified. Of six Ad-2-transformed epithelial lines isolated from clone C3 only one was tumourigenic in syngeneic rats, whereas all six transformants produced tumours in athymic nude mice. There was a remarkable variation in the morphology of the Ad-2-transformed liver cells, ranging from an epithelial morphology similar to C3 cells to cells with a distinct lymphoid morphology. The in vitro and in vivo behaviour of the Ad-2-transformed clone C3 cells reported in this communication, taken together with our previous report on the characteristics of Ad-12-transformed C3 cells, clearly show that the differences observed between Ad-2- and Ad-12-transformed rat embryo cells were also observed in our studies using cloned rat liver epithelial cultures. Our findings clearly rule out the hypothesis that the heterogeneity of Ad-2 transformation events is the result of the transformation in vitro of different types of target cell. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-58-1-73