1887

Abstract

SUMMARY

Intracellular nucleocapsids, virtually free of host proteins and nucleic acids, were purified from cells acutely infected with several strains of mumps virus. Viral RNA obtained from the nucleocapsids was predominantly a single 50S species, with 28S RNA occurring as a secondary peak in the RNA profile. Nucleocapsids yielded approx. 9 µg 50S RNA per 10 infected cells. The 50S RNA was partially self-complementary, indicating that mumps viral nucleocapsids include full-length plus (+) sense antigenomes. Self-annealing and competition hybridization assays with 50S RNA suggest that 28S RNA includes duplexes consisting of full-length (+) and minus (-) sense 50S RNA. Properties of the viral RNA did not vary with the method of RNA isolation, time post-infection at which nucleocapsids were harvested, nor the strain of mumps virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-58-1-205
1982-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/58/1/JV0580010205.html?itemId=/content/journal/jgv/10.1099/0022-1317-58-1-205&mimeType=html&fmt=ahah

References

  1. Azumi P. H. 1969; Mumps meningoencephalitis in children. Journal of the American Medical Association 207:509–512
    [Google Scholar]
  2. Baltimore D. 1966; Purification and properties of poliovirus double-stranded ribonucleic acid. Journal of Molecular Biology 18:421–428
    [Google Scholar]
  3. Bang H. O., Bang J. 1943; Involvement of the central nervous system in mumps. Acta Medica Scandinavica 113:487–505
    [Google Scholar]
  4. East J. L., Kingsbury D. W. 1971; Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells. Journal of Virology 8:161–173
    [Google Scholar]
  5. Enders J. F., Levens J. H., Stokes J. Jr, Maris E. P., Berenberg W. 1946; Attenuation of virulence with retention of antigenicity of mumps virus after passage in the embryonated egg. Journal of Immunology 54:283–291
    [Google Scholar]
  6. Hall W. W., Ter Meulen V. 1977; Polyadenylic acid [poly(A)] sequences associated with measles virus intracellular ribonucleic acid (RNA) species. Journal of General Virology 35:497–510
    [Google Scholar]
  7. Kolakofsky D., Bruschi A. 1975; Antigenomes in Sendai virions and Sendai virus-infected cells. Virology 66:185–191
    [Google Scholar]
  8. Kolakofsky D., De La Tour E. B., Bruschi A. 1974; Self-annealing of Sendai virus RNA. Journal of Virology 14:33–39
    [Google Scholar]
  9. Lamb R. A., Choppin P. W. 1978; Determination by peptide mapping of the unique polypeptides in Sendai virions and infected cells. Virology 84:469–478
    [Google Scholar]
  10. Mccarthy M., Johnson R. T. 1980a; A comparison of the structural polypeptides of five strains of mumps virus. Journal of General Virology 46:15–27
    [Google Scholar]
  11. Mccarthy M., Johnson R. T. 1980b; Morphologic heterogeneity in relation to structural and functional properties of mumps virus. Journal of General Virology 48:395–397
    [Google Scholar]
  12. Mccarthy M., Jubelt B., Fay D., Johnson R. T. 1980; Comparative studies of five strains of mumps virus in vitro and in neonatal hamsters: evaluation of growth, cytopathogenicity, and neurovirulence. Journal of Medical Virology 5:1–15
    [Google Scholar]
  13. Mcfarlin D. E., Bellini W. J., Mingioli E. S., Behar T. N., Trudgett A. 1980; Monospecific antibody to the haemagglutinin of measles virus. Journal of General Virology 48:425–429
    [Google Scholar]
  14. Miller C. A., Raine C. S. 1979; Heterogeneity of virus particles in measles virus. Journal of General Virology 45:441–453
    [Google Scholar]
  15. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of Simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 6:677–684
    [Google Scholar]
  16. Robinson W. S. 1970; Self-annealing of subgroup 2 myxovirus RNA’s. Nature, London 225:944–945
    [Google Scholar]
  17. Soria M., Little S. P., Huang A. S. 1974; Characterization of vesicular stomatitis nucleocapsids. I. Complementary 40S RNA molecules in nucleocapsids. Virology 61:270–280
    [Google Scholar]
  18. Sprague J., Eron L. J., Seifried A. S., Milstein J. B. 1979; Cell-free synthesis of measles virus proteins. Journal of Virology 32:688–691
    [Google Scholar]
  19. Stallcup K. C., Wechsler S. L., Fields B. N. 1979; Purification of measles virus and characterization of subviral components. Journal of Virology 30:166–176
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-58-1-205
Loading
/content/journal/jgv/10.1099/0022-1317-58-1-205
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error