1887

Abstract

SUMMARY

The virion polypeptide composition of three independently isolated tree shrew herpesviruses (THV) was analysed by SDS-polyacrylamide slab gel electrophoresis and by a two-dimensional technique using isoelectric focusing. Two of the virus isolates analysed were from malignant tumours; the other isolate (THV, strain 1) was from an apparently healthy animal. The polypeptide patterns of the three purified Tupaia herpesvirus isolates were remarkably similar, each consisting of at least 35 polypeptides ranging in mol. wt. from 12000 to 230000. Whilst the majority of analogous polypeptides of the three viruses were of indistinguishable electrophoretic mobility, some (e.g. polypeptides of 82K to 86K) showed small differences in apparent mol. wt. which were characteristic of the virus strain. Comparative SDS-polyacrylamide gel electrophoresis made it possible to distinguish the Tupaia herpesvirus isolates from each other. At least five glycoproteins were found in purified THV virions. The two-dimensional electropherograms revealed at least 47 discernible protein spots, some of which were specific for a given THV isolate and which were detectable even in lysates of THV-infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-58-1-139
1982-01-01
2022-07-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/58/1/JV0580010139.html?itemId=/content/journal/jgv/10.1099/0022-1317-58-1-139&mimeType=html&fmt=ahah

References

  1. Allen G. P., Randall C. C. 1979; Proteins of equine herpes virus type 3.1. Polypeptides of the purified virion. Virology 92:252–257
    [Google Scholar]
  2. Chousterman S., Lacasa M., Sheldrick P. 1979; Physical map of the channel catfish virus genomes: location of sites for restriction endonucleases Eco RI, Hind III, Hpa I, and Xba I. Journal of Virology 31:73–85
    [Google Scholar]
  3. Darai G., Matz B., Schröder C. H., Flügel R. M., Berger U., Munk K., Gelderblom H. 1979; Characterization of a tree shrew herpesvirus isolated from a lymphosarcoma. Journal of General Virology 32:341–351
    [Google Scholar]
  4. Darai G., Matz B., Flügel R. M., Delius H. 1981; DNA of Tupaia herpesviruses. In Herpesvirus DNA Edited by Becker Y. The Hague: Martinus NyhofT; (in press)
    [Google Scholar]
  5. Dixon A. F., Farber F. E. 1980; Channel catfish virus: physico-chemical properties of the viral genome and identification of viral polypeptides. Virology 103:267–278
    [Google Scholar]
  6. Essani K., Dales S. 1979; Biogenesis of vaccinia: evidence for more than 100 polypeptides in the virion. Virology 95:385–394
    [Google Scholar]
  7. Fiedler W., Hofer H., Schulz A. H., Starck D. 1956; Übersicht über das System der Primaten. Primatology 1:1–252
    [Google Scholar]
  8. Given D., Kieff E. 1978; DNA of Epstein-Barr virus. IV. Linkage maps of restriction endonuclease fragments of the B95-8 and W91 strains of Epstein-Barr virus. Journal of Virology 28:524–542
    [Google Scholar]
  9. Haar L., Marsden H. S. 1981; Two-dimensional gel analysis of HSV type 1-induced polypeptides and glycoprotein processing. Journal of General Virology 52:77–92
    [Google Scholar]
  10. Hayward S. D., Nogee L., Hayward G. S. 1980; Organization of repeated regions within the Epstein-Barr virus DNA molecule. Journal of Virology 33:507–521
    [Google Scholar]
  11. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  12. Hofmann W., Möller P., Schwaier A., Flügel R. M., Zöller L., Darai G. 1981; Malignant tumours in Tupaia (tree shrew). Journal of Medical Primatology (in press)
    [Google Scholar]
  13. Honess R. W., Watson D. H. 1977; Unity and diversity in the herpesviruses. Journal of General Virology 37:15–37
    [Google Scholar]
  14. Killington R. A., Yeo J., Honess R. W., Watson D. H., Halliburton J. W., Mumford J. 1977; Comparative analysis of the proteins and antigens of five herpesviruses. Journal of General Virology 37:297–310
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  17. Lawrence W. C. 1976; Purification of equine herpes virus type 1. Journal of General Virology 31:81–91
    [Google Scholar]
  18. Mccombs R. M., Brunschwig J. P., Mirkovic R., Benyesh-Melnick M. 1971; Electron microscopic characterization of a herpes-like virus isolated from tree shrews. Virology 45:816–820
    [Google Scholar]
  19. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  20. Mirkovic R., Voss W. R., Benyesh-Melnick M. 1970; Characterization of a new herpes-type virus indigenous for tree shrews. Proceedings of the 10th International Congress of Microbiology Mexico City pp 181–189
    [Google Scholar]
  21. Moore W. G., Goodman M. 1968; A set theoretical approach in immunotaxonomy. Analysis of species comparison in modified Ouchterlony plates. Bulletin of Mathematical Biophysics 30:279–289
    [Google Scholar]
  22. O’farrell P. H. 1975; High-resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  23. Roizman B. 1979; The organization of the herpes simplex virus genomes. Annual Review of Genetics 13:25–57
    [Google Scholar]
  24. Subak-Sharpe J. H., Brown S. M., Ritchie D. A., Timbury M. C., Macnab J. C. M., Marsden H. S., Hay J. 1974; Genetic and biochemical studies with herpesviruses. Cold Spring Harbor Symposia on Quantitative Biology 39:717–730
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-58-1-139
Loading
/content/journal/jgv/10.1099/0022-1317-58-1-139
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error