%0 Journal Article %A Robertson, James S. %A Etchison, James R. %A Summers, Donald F. %T Comparison of the Oligosaccharide Structure of the Glycoprotein of Vesicular Stomatitis Virus and a Thermolabile Mutant (tl-17) %D 1982 %J Journal of General Virology, %V 58 %N 1 %P 13-23 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-58-1-13 %K oligosaccharide structure %K VSV(tl-17) %K glycoprotein %I Microbiology Society, %X SUMMARY As a means of examining the extent to which the polypeptide structure of a virus glycoprotein contributes to the overall structure and composition of the carbohydrate moieties, we have made a detailed comparison of the structure of the oligosaccharide moieties of wild-type vesicular stomatitis virus (VSV) glycoprotein with those of a glycoprotein-defective mutant of VSV, tl-17 (VSV). Characterization of the oligosaccharides by ion-exchange and gel filtration chromatography after sequential enzymic degradation reveals similar structures in the wt and mutant glycoproteins. However, the altered polypeptide structure of the tl-17 glycoprotein affects the extent of addition of sialic acid and fucose, both of which are added late in the maturation of the glycoprotein. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-58-1-13