1887

Abstract

SUMMARY

Astrovirus could be serially passed at least 13 times in primary human embryo kidney (HEK) cells when 10 µg/ml of crystalline trypsin was incorporated in a serum-free maintenance medium. In the presence of trypsin the virus was also passed and adapted to a continuous line of rhesus monkey kidney cells (LLCMK) and primary baboon kidney (PBK) cells in which it was passed 25 and 16 times respectively, without evidence of diminishing infectivity. Attempts to adapt the virus to other cell lines (Vero, Hep II, MRC-5, BHK and HRT-18) were unsuccessful. After 11 passages in HEK cells, a titration of virus grown in different concentrations of trypsin showed that virus propagation was still trypsin-dependent.

Keyword(s): astrovirus , tissue culture and trypsin
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/content/journal/jgv/10.1099/0022-1317-57-2-421
1981-12-01
2022-01-28
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References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin on the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Ashley C. R., Caul E. O., Paver W. K. 1978; Astrovirus-associated gastroenteritis in children. Journal of Clinical Pathology 31:939–943
    [Google Scholar]
  3. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  4. Banatvala J. E., Totterdell B. M., Chrystie I. L., Woode G. N. 1975; In vitro detection of human rotaviruses. Lancet ii:821
    [Google Scholar]
  5. Graham D. Y., Estes M. K. 1980; Proteolytic enhancement of rotavirus infectivity: biologic mechanisms. Virology 101:432–439
    [Google Scholar]
  6. Kurtz J. B., Lee T. W., Pickering D. 1977; Astrovirus-associated gastroenteritis in a children’s ward. Journal of Clinical Pathology 30:948–952
    [Google Scholar]
  7. Kurtz J. B., Lee T. W., Craig J. W., Reed S. E. 1979; Astrovirus infection in volunteers. Journal of Medical Virology 3:221–230
    [Google Scholar]
  8. Lee T. W., Kurtz J. B. 1977; Astroviruses detected by immunofluorescence. Lancet ii:406
    [Google Scholar]
  9. Madeley C. R., Cosgrove B. P. 1975; 28 nm particles in faeces in infantile gastroenteritis. Lancet ii:451–452
    [Google Scholar]
  10. Spendlove R. S., Schaeffer F. L. 1965; Enzymatic enhancement of infectivity of reovirus. Journal of Bacteriology 89:597–602
    [Google Scholar]
  11. Spendlove R. S., Mcclain M. E., Lennette E. M. 1970; Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes. Journal of General Virology 8:83–94
    [Google Scholar]
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