1887

Abstract

SUMMARY

The double-stranded (ds)RNA components of two serologically related viruses (019/6-A and 38-4-A), obtained from , have been compared. 019/6-A virus has two RNA components, ds1 and ds2, whereas 38-4-A virus has three RNA components, ds1, ds2 and ds3. T1 oligonucleotide fingerprint analysis and saturation hybridization experiments indicated that 019/6-A ds1 and 38-4-A ds1 were very similar, whereas 019/6-A ds2 and 38-4-A ds2 were about 50% homologous. 38-4-A ds3 had little homology with ds1 or ds2 of either virus. The single-stranded (ss)RNA transcripts of 38-4-A virus ds1, ds2 and ds3 each directed the synthesis of a single polypeptide, with mol. wt. 62000, 55000 and 52000 respectively. Peptide mapping showed that these three polypeptides were unrelated in sequence and that ds2 encoded the virus capsid polypeptide. The ds3 is probably not required for the replication of 38-4-A virus and may be regarded as a satellite RNA.

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1981-12-01
2024-12-07
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References

  1. Bevan E. A., Mitchell D. J. 1979; Killer systems of yeast. In Viruses and Plasmids in Fungi pp. 161–199 Edited by Lemke P. A. New York: Marcel Dekker;
    [Google Scholar]
  2. Bruenn J. A., Brennan V. E. 1980; Yeast viral double-stranded RNAs have heterogeneous 3′ termini. Cell 19:923–933
    [Google Scholar]
  3. Bruenn J. A., Kane W. 1978; The relatedness of the double-stranded RNAs present in yeast virus-like particles. Journal of Virology 26:762–772
    [Google Scholar]
  4. Buck K. W. 1980; Viruses and killer factors of fungi. In The Eukaryotic Microbial Cell. Symposia of the Society for General Microbiology 30:329–375
    [Google Scholar]
  5. Buck K. W., Ratti G. 1975; Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. Journal of General Virology 27:211–224
    [Google Scholar]
  6. Buck K. W., Ratti G. 1977; Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components. Journal of General Virology 37:215–219
    [Google Scholar]
  7. Buck K. W., Almond M. R., Mcfadden J. J. P., Romanos M. A., Rawlinson C. J. 1981a; Properties of thirteen viruses and virus variants obtained from eight isolates of the wheat take-all fungus, Gaeumannomyces graminis var. tritici. Journal of General Virology 53:235–245
    [Google Scholar]
  8. Buck K. W., Romanos M. A., Mcfadden J. J. P., Rawlinson C. J. 1981b; In vitro transcription of double-stranded RNA by virion-associated RNA polymerases of viruses from Gaeumannomyces graminis. Journal of General Virology 51:157–168
    [Google Scholar]
  9. Day P. R., Dodds J. A. 1979; Viruses of plant pathogenic fungi. In Viruses and Plasmids in Fungi pp. 201–238 Edited by Lemke P. A. New York: Marcel Dekker;
    [Google Scholar]
  10. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  11. Gould A. R., Symons R. H. 1977; Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4:3787–3802
    [Google Scholar]
  12. Gunn M. R., Symons R. H. 1980; Sequence homology at the 3′-termini of the four RNAs of alfalfa mosaic virus. FEBS Letters 109:145–150
    [Google Scholar]
  13. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology pp. 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  14. Kane W. P., Pietras D. F., Bruenn J. A. 1979; The evolution of defective-interfering dsRNAs of the yeast killer virus. Journal of Virology 32:692–696
    [Google Scholar]
  15. Kennedy S. I. T. 1976; Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. Journal of Molecular Biology 108:491–511
    [Google Scholar]
  16. Koltin Y. 1977; Virus-like particles in Ustilago maydis: mutants with partial genomes. Genetics 86:527–534
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  19. Lilly V. G., Barnett H. L. 1951 Physiology of the Fungi p 426 New York: McGraw-Hill;
    [Google Scholar]
  20. Loviny T., Szekely M. 1973; Fingerprinting double-stranded non-radioactive RNA from a fungal virus. European Journal of Biochemistry 35:87–94
    [Google Scholar]
  21. Morrison T. G., Lodish H. F. 1975; Site of synthesis of membrane and non-membrane proteins of vesicular stomatitis virus. Journal of Biological Chemistry 250:6955–6962
    [Google Scholar]
  22. Mossop D. W., Francki R. I. B. 1978; Survival of a satellite RNA in vivo and its dependence on cucumber mosaic virus for its replication. Virology 86:562–566
    [Google Scholar]
  23. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  24. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  25. Pinck L., Pinck M. 1979; Sequence homology at the 3′-ends of alfalfa mosaic virus RNAs. FEBS Letters 107:61–65
    [Google Scholar]
  26. Ratti G., Buck K. W. 1972; Virus particles in Aspergillus foetidus: a multicomponent system. Journal of General Virology 14:165–175
    [Google Scholar]
  27. Schuerch A. R., Mitchell W. R., Joklik W. K. 1975; Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis. Analytical Biochemistry 65:331–345
    [Google Scholar]
  28. Symons R. H. 1979; Extensive sequence homology at the 3′-termini of the four RNAs of cucumber mosaic virus. Nucleic Acids Research 7:825–837
    [Google Scholar]
  29. Vandewalle M. J., Siegel A. 1976; A study of nucleotide sequence homology between strains of tobacco mosaic virus. Virology 73:413–418
    [Google Scholar]
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