1887

Abstract

SUMMARY

Model systems to study restricted primate retrovirus expression were established by infection of canine foetal thymus cells (CF-2Th) and superinfection of HEL-12 cells with HEL-12 virus. In the resulting CF-2Th/HEL-12V cells and HEL-12/HEL-12V cells, four sequential stages of virus infection were defined by the production of reverse transcriptase (RT)-containing particles and expression of virus antigens as detected by radioimmunoassays. Stage 1 cells did not synthesize virus antigens or produce RT-containing particles. Stage 2 cells synthesized virus antigen but not RT-containing particles. Stage 3 cells synthesized antigen and produced RT-containing particles, and stage 4 cells synthesized virus antigens but no longer produced RT-containing particles. The duration of the four stage infection is 2 to 3 weeks in both cell types. Monospecific competition radioimmunoassays to detect HEL-12V p30 or gp70 antigen showed high levels of virus antigen throughout stages 2 to 4 of infection. Analysis of immunoprecipitates formed under conditions to detect either p30- or gp70-containing proteins in cells pulsed and pulsed-chased with [H]eucine showed the same spectrum of virus precursor polyproteins, intermediates and mature virion components in stage 2 to 4 cells in canine and human infections. Spent culture fluids collected from stage 3 and stage 4 CF-2Th/HEL-12V cells failed to reveal inhibitors of RT activity. Stage 4 CF-2Th/HEL-12V or HEL-12/HEL-12V cells labelled with [H]uridine produced virions which incorporated [H]uridine but did not have RT activity, suggesting that restricted infection is characterized by production of HEL-12V defective in RT activity.

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1981-12-01
2021-10-22
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References

  1. Bandyopadhyay A. K., Chang E. H., Levy C. C., Friedman R. M. 1979; Structural abnormalities in murine leukemia viruses produced by interferon treated cells. Biochemical and Biophysical Research Communications 87:983–988
    [Google Scholar]
  2. Barre-Sinoussi F., Montagnier L., Lidereau R., Sisman J., Wood J., Chermann J. C. 1979; Enhancement of retrovirus production by anti-interferon serum. Annales de Microbiology 130B:349–362
    [Google Scholar]
  3. Bergholz C. M., Wolfe L. G., Deinhardt F., Thakkar B., Marczynska B. 1977; Oncogenicity in marmosets of HL-23V, a type-C oncornavirus isolated from human leukemic cells, and comparison with a simian sarcoma virus type-1(SSAV-l/SSAV-1). Journal of the National Cancer Institute 58:1041–1045
    [Google Scholar]
  4. Bergholz C. M., Reynolds J. T., Panem S. 1980; Biological and antigenic characteristics of HEL-12 virus. Journal of General Virology 50:247–258
    [Google Scholar]
  5. Berkower A. S., Lilly F., Soeiro R. 1980; Expression of viral RNA in Friend virus-induced erythroleukemia cells. Cell 19:637–642
    [Google Scholar]
  6. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  7. Hefti E., Reynolds J. T., Panem S., Kirsten W. H. 1980; Characterization of unique nucleotide sequences and antigenic determinants of a type-C virus isolated from normal human cells (HEL-12 virus). In Viruses in Naturally Occurring Cancers pp. 729–736 New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 221:680–685
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Markham P. D., Ruscetti F., Salahuddin S. Z., Gallagher R. E., Gallo R. C. 1979; Enhanced induction of growth of B lymphoblasts from fresh human blood by primate type-C retroviruses (gibbon ape leukemia virus and simian sarcoma virus). International Journal of Cancer 23:148–156
    [Google Scholar]
  11. Nelson J. A., Levy J. A., Leong J. C. 1981; Human placentas contain a specific inhibitor of RNA-directed DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 78:1670–1674
    [Google Scholar]
  12. Panem S. 1978; Spontaneous type C virus expression in human embryonic lung cells (HEL-12): comparison of cloned and mixed cell populations. In Animal Virology pp. 409–417 Edited by Baltimore D., Huang A., Fox C. F. New York: Academic Press;
    [Google Scholar]
  13. Panem S., Prochownik E. V., Reale F. R., Kirsten W. H. 1975; Isolation of type-C virions from a normal human fibroblast strain. Science 189:297–299
    [Google Scholar]
  14. Panem S., Ordonez N. G., Kirsten W. H., Katz A. L., Spargo B. H. 1976; C-type virus expression in systemic lupus erythematosus. New England Journal of Medicine 295:470–475
    [Google Scholar]
  15. Panem S., Prochownik E. V., Knish W. M., Kirsten W. H. 1977; Cell generation and type-C virus expression in the human embryonic cell strain HEL-12. Journal of General Virology 35:487–495
    [Google Scholar]
  16. Pitha P. M., Fernie B., Maldarelli F., Hattman T., Wivel N. A. 1980; Effect of interferon on mouse leukemia virus (MuLV) V. Abnormal proteins in virions of Rauscher MuLV produced in the presence of interferon. Journal of General Virology 46:97–110
    [Google Scholar]
  17. Prochownik E. V., Kirsten W. H. 1976; Inhibition of reverse transcriptase of primate type-C viruses by 7S immunoglobulin from patients with leukemia. Nature, London 260:64–67
    [Google Scholar]
  18. Prochownik E. V., Kirsten W. H. 1977; Nucleic acid sequences of primate type-C viruses in normal and neoplastic human tissues. Nature, London 267:175–177
    [Google Scholar]
  19. Prochownik E. V., Panem S., Kirsten W. H. 1979; Type-C virus induced by iododeoxyuridine in the human embryonic cell strain HEL-12. Journal of General Virology 42:399–403
    [Google Scholar]
  20. Reynolds J. T., Panem S. 1981; Characterization of antibody to C-type virus antigens isolated from immune complexes in kidneys of patients with systemic lupus erythematosus. Laboratory Investigation 44:410–419
    [Google Scholar]
  21. Shields A., Witte O. N., Rothenberg E., Baltimore D. 1978; High frequency of aberrant expression of Moloney murine leukemia virus in clonal infections. Cell 14:601–609
    [Google Scholar]
  22. Stephenson J. R. (editor) 1980 Molecular Biology of RNA Tumor Viruses New York: Academic Press;
    [Google Scholar]
  23. Toyoshima K., Vogt P. K. 1969; Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology 38:414–426
    [Google Scholar]
  24. Whiteley S. A., Naso R. B. 1981; A comparative analysis: the intracellular precursor polyproteins of baboon endogenous retroviruses and the human viral isolate HL23V. Journal of Virology (in press)
    [Google Scholar]
  25. Wu A. M., Schulte A., Gallo R. C. 1976; Synthesis of type C virus particles from murine cultured cells induced by iododeoxyuridine. V. Effect of interferon and its interaction with dexamethasone. Journal of Virology 19:108–117
    [Google Scholar]
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