1887

Abstract

SUMMARY

We have used cloned fragments of Marek’s disease virus (MDV) DNA and hybridization to search for virus DNA and study its expression in infected chick embryo fibroblasts (CEF), lymphoblastoid cell lines, tumours and neural lesions. DNA from the HPRS 16/att strain of MDV was cleaved with RI endonuclease and several fragments were cloned in using the vector PBR322. Seven fragments ranging in size from 2.6 to 11 kbp representing approx. 25% of the MDV genome were labelled and annealed to RI digests of DNA from infected cells and tumours following separation and transfer according to the Southern blotting procedure. Most of the selected MDV DNA fragments hybridized to fragments of corresponding sizes in RI digests of DNA from cell lines and tumours and failed to hybridize to digests of uninfected chick cell DNA. hybridization using H-labelled DNA with specific activity of 10 d/min/µg as probe showed intranuclear MDV DNA in infected CEF, in every cell of two lymphoblastoid cell lines and in the majority of infiltrating or proliferating lymphoid cells found in type ‘A’ lesions of grossly enlarged peripheral nerves. Both intranuclear and cytoplasmic RNA were detected in cells that contained virus DNA. However, comparatively little virus RNA appears to be transcribed in cell lines and in infected tissues from the regions of virus DNA (25% of genome) used as probe in this study. Our results favour the hypothesis that the accumulation of lymphoid cells in nerves is not the result of an inflammatory response to infected nerve cells but is rather the consequence of proliferating transformed cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-57-2-285
1981-12-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/57/2/JV0570020285.html?itemId=/content/journal/jgv/10.1099/0022-1317-57-2-285&mimeType=html&fmt=ahah

References

  1. Akiyama V., Kato S., Iwa N. 1973; Continuous cell culture from lymphoma of Marek’s disease. Biken Journal 16:177–179
    [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coll. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  3. Brahic M., Haase A. T. 1978; Detection of viral sequences of low reiteration frequency by in situ hybridization. Proceedings of the National Academy of Sciences of the United States of America 75:6125–6129
    [Google Scholar]
  4. Brahic M., Stowring L., Ventura P., Haase A. T. 1981; Gene expression in visna virus infection in sheep. Nature, London 292:240–242
    [Google Scholar]
  5. Churchill A. E. 1968; Herpes-type virus isolated in cell culture from tumours of chickens with Marek’s disease. I. Studies in cell culture. Journal of the National Cancer Institute 41:939–950
    [Google Scholar]
  6. Churchill A. E., Biggs P. M. 1967; Agent of Marek’s disease in tissue culture. Nature, London 215:528–530
    [Google Scholar]
  7. Churchill A. E., Chubb R. C., Baxendale W. 1969; The attenuation with loss of oncogenicity of the herpes-type virus of Marek’s disease (strain HPRS 16) on passage in cell culture. Journal of General Virology 4:557–564
    [Google Scholar]
  8. Clewell O. B., Helinski D. A. 1969; Supercoil circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proceedings of the National Academy of Sciences of the United States of America 62:1159–1166
    [Google Scholar]
  9. Delorbe W. J., Lucuw P. A., Goodman H. M., Varmus H. E., Bishop J. M. 1980; Molecular cloning and characterization of avian sarcoma virus circular DNA molecule. Journal of Virology 36:50–61
    [Google Scholar]
  10. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of A merica 72:1184–1188
    [Google Scholar]
  11. Haase A. T., Ventura P., Gibbs C. J. Jr, Tourtelotte W. W. 1981; Measles virus nucleotide sequences: detection by hybridization in situ. Science 212:672–674
    [Google Scholar]
  12. Hughes S. H., Stubblefield E., Nazerian K., Varmus H. E. 1980; DNA of a chicken herpesvirus is associated with at least two chromosomes in a chicken lymphoblastoid cell line. Virology 105:234–240
    [Google Scholar]
  13. Kaschka-Dierich C., Nazerian K., Thomssen R. 1979; lntracellular state of Marek’s disease virus DNA in two lymphoblastoid tumour-derived chicken cell lines. Journal of General Virology 44:271–280
    [Google Scholar]
  14. Lawn A. M., Payne L. N. 1979; Chronological study of ultrastructural changes in the peripheral nerves in Marek’s disease. Neuropathology and Applied Neurobiology 5:485–497
    [Google Scholar]
  15. Nazerian K., Lee L. F. 1974; DNA of Marek’s disease virus in a lymphoblastoid cell line from Marek’s disease tumours. Journal of General Virology 25:317–321
    [Google Scholar]
  16. Nazerian K., Lindahl T., Klein G., Lee L. F. 1973; Deoxyribonucleic acid of Marek’s disease virus in virus-induced tumours. Journal of Virology 12:841–846
    [Google Scholar]
  17. Nazerian K., Stephens E. A., Sharma J. M., Lee L. F., Gailitis M., Witter R. L. 1977; A non-producer T lymphoblastoid cell line from Marek’s disease transplantable tumor (JMV). Avian Diseases 21:69–76
    [Google Scholar]
  18. Payne L. N., Biggs P. M. 1967; Studies on Marek’s disease. II. Pathogenesis. Journal of the National Cancer Institute 39:281–302
    [Google Scholar]
  19. Payne L. N., Frazier J. A., Powell P. C. 1976; Pathogenesis of Marek’s disease. International Review of Experimental Pathology 16:59–154
    [Google Scholar]
  20. Powell E. C., Payne L. N., Prazier J. A., Rennin M. 1974; T lymphoblastoid cell lines from Marek’s disease lymphomas. Nature, London 251:79–81
    [Google Scholar]
  21. Purchase R. G., Biggs P. M. 1967; Characterization of five isolates of Marek’s disease. Research in Veterinary Science 8:440–449
    [Google Scholar]
  22. Ross L. J. N., Biggs P. M., Newton A. A. 1973; Purification and properties of the ‘A’ antigen associated with Marek’s disease virus infections. Journal of General Virology 18:291–304
    [Google Scholar]
  23. Ross L. J. N., Powell P. C., Walker D. J., Rennie M., Payne L. N. 1977; Expression" of virus-specific, thymus-specific and tumour-specific antigens in lymphoblastoid cell lines derived from Marek’s disease lymphomas. Journal of General Virology 35:2192013235
    [Google Scholar]
  24. Shank P. R., Hughes S. H., Kung H. J., Majors R. V., Quintrell J. E., Guntaka N., Bishop J. M., Varmus H. E. 1978; Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in the species of circular DNA. Cell 15:1383–1395
    [Google Scholar]
  25. Silver S., Tanaka A., Nonoyama M. 1979; Transcription of the Marek’s disease virus genome in a non-productive chicken lymphoblastoid cell line. Virology 95:127–133
    [Google Scholar]
  26. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  27. Tanaka A., Silver S., Nonoyama M. 1978; Biochemical evidence of the non-integrated status of Marek’s disease virus DNA in transformed lyrnphoblastoid cells of chicken. Virology 88:19–24
    [Google Scholar]
  28. Witter R. L., Stephens E. A., Sharma J. M., Nazerlan K. 1975; Demonstration of a tumour-associated surface antigen in Marek’s disease. Journal of Immunology 115:177–183
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-57-2-285
Loading
/content/journal/jgv/10.1099/0022-1317-57-2-285
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error