1887

Abstract

SUMMARY

Syncytia formation induced by the bovine leukaemia virus (BLV) has been classified as ‘early’ or ‘late’ polykaryocytosis. Early polykaryocytosis arises in the first 24 h in mixed cultures of BLV-infected cells with indicator cells. Late polykaryocytosis is observed 4 to 8 days after infection of sensitive cells with cell-free infectious BLV. Our results demonstrate that the two phenomena proceed from different mechanisms. Late polykaryocytosis results from an active process dependent on the integrity of the virus genome. In contrast, early polykaryocytosis is a passive process which does not require any de novo virus synthesis but is dependent on the presence of virus proteins in inducer cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-57-1-227
1981-11-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/57/1/JV0570010227.html?itemId=/content/journal/jgv/10.1099/0022-1317-57-1-227&mimeType=html&fmt=ahah

References

  1. Astier T., Mamoun R., Guillemain B., Parodi A. L., Duplan J. F. 1978; Immunological nature of the inhibition of BLV-induced early polykaryocytosis by bovine sera. International Journal of Cancer 22:47–54
    [Google Scholar]
  2. Bassin R. H., Tuttle N., Fischinger P. J. 1971; Rapid cell culture assay technique for murine leukaemia viruses. Nature, London 229:564–566
    [Google Scholar]
  3. Benton C. V., Soria A. E., Gilden R. V. 1978; Direct syncytial assay for the quantitation of bovine leukemia virus. Infection and Immunity 20:307–309
    [Google Scholar]
  4. Cornefert-Jensen F., Hare W. C., Stock N. D. 1969; Studies on bovine lymphosarcoma: formation of syncytia and detection of virus particles in mixed cell cultures. International Journal of Cancer 4:507–519
    [Google Scholar]
  5. Ferrer J. F., Cabradilla C. D. 1978; The phenomenon of polykaryocytosis induced by BLV in mixed cultures: specificity, mechanisms and application to the diagnosis of BLV infection in cattle. Annales de Recherches Veterinaires 9:721–728
    [Google Scholar]
  6. Ferrer J. F., Diglio C. 1976; Development of an in vitro infectivity assay for the C-type bovine leukemia virus. Cancer Research 36:1068–1073
    [Google Scholar]
  7. Ghysdael J., Kettmann R., Burny A. 1979; Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems. Journal of Virology 29:1087–1098
    [Google Scholar]
  8. Greig A. S., Chander S., Samagh B., Bouillant A. M. P. 1978; A simple, rapid syncytial inhibition test for antibodies to bovine leukemia virus. Canadian Journal of Comparative Medicine 42:446–451
    [Google Scholar]
  9. Guillemain B., Mamoun R., Levy D., Astier T., Parodi A. L. 1977a; Early polykaryocytosis inhibition (EPI): a simple and quantitative test for the detection of bovine leukemia virus infection in cattle. In Bovine Leucosis: Various Methods of Molecular Virology pp 337–346 Edited by Burny A. Luxembourg: Commission of European Communities;
    [Google Scholar]
  10. Guillemain B., Mamoun R., Levy D., Astier T., Irgens K., Parodi A. L. 1978; Early polykaryocytosis inhibition: a simple quantitative in vitro assay for the detection of bovine leukemia virus infection in cattle. European Journal of Cancer 14:811–827
    [Google Scholar]
  11. Irgens K., Pinelli C., Guillemain B., Levy D., Parodi A. L. 1977; Early syncytium formation induced by bovine leukemia virus in mixed cultures. Biomedicine 27:49–50
    [Google Scholar]
  12. Kawakami T. G., Moore A. L., Theilen G. H., Munn R. J. 1970; Comparisons of virus-like particles from leukotic cattle to feline leukosis virus. Bibliotheca Haematologica 36:471–475
    [Google Scholar]
  13. Kettmann R., Burny A., Cleuter Y., Ghysdael J., Mammerickx M. 1978; Distribution of bovine leukemia virus proviral DNA sequences in tissues of animals with enzootic bovine leucosis.
    [Google Scholar]
  14. Latarjet R., Cramer R., Montagnier L. 1967; Inactivation by UV-, X-, and y-radiations, of the infecting and transforming capacities of polyoma virus. Virology 33:104–111
    [Google Scholar]
  15. Miller J. M., Miller L. D., Olson C., Gillette K. G. 1969; Virus-like particles in phytohemagglutinin stimulated lymphocyte cultures with reference to bovine lymphosarcoma. Journal of the National Cancer Institute 43:1297–1305
    [Google Scholar]
  16. Nomura S., Bassin R. H., Turner W., Haapala D. K., Fischinger P. J. 1972; Ultraviolet inactivation of Moloney leukemia virus: relative target size required for virus replication and rescue of ‘defective’ murine sarcoma virus. Journal of General Virology 14:213–217
    [Google Scholar]
  17. Ogura H., Paulsen J., Bauer H. 1977; Cross-neutralization of ovine and bovine C-type leukemia virus-induced syncytia formation. Cancer Research 37:1486–1489
    [Google Scholar]
  18. Onuma M., Watarai S., Mikami T., Izawa H. 1980; Cell fusion activity of bovine leukaemia virus. Journal of General Virology 48:421–424
    [Google Scholar]
  19. Poste G. 1970; Virus-induced polykaryocytosis and the mechanism of cell fusion. Advances in Virus Research 16:303–356
    [Google Scholar]
  20. Van Der Maaten M. J., Miller J. M. 1976; Replication of bovine leukemia virus in monolayer cell cultures. Bibliotheca Haematologica 43:360–362
    [Google Scholar]
  21. Van Der Maaten M. J., Miller J. M. 1977; An evaluation of the syncytium assay for the detection of bovine leukemia virus in peripheral blood leukocytes. In Bovine Leucosis: Various Methods of Molecular Virology pp 299–307 Edited by Burny A. Luxembourg: Commission of European Communities;
    [Google Scholar]
  22. Van Der Maaten M. J., Miller J. M., Boothe A. D. 1974; Replicating type-C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma. Journal of the National Cancer Institute 52:491–497
    [Google Scholar]
  23. Yoshikura H. 1971; Ultraviolet inactivation of murine leukemia and sarcoma viruses. International Journal of Cancer 1:131–140
    [Google Scholar]
  24. Yoshikura H. 1973; Ultraviolet sensitivity of helper function of murine leukemia virus. Archives of Biochemistry and Biophysics 154:76–83
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-57-1-227
Loading
/content/journal/jgv/10.1099/0022-1317-57-1-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error