%0 Journal Article %A Murray, J. D. %A Berger, M. L. %A Taylor, I. W. %T Flow Cytometric Analysis of DNA Content of Mouse Liver Cells Following in vivo Infection by Human Adenovirus Type 5 %D 1981 %J Journal of General Virology, %V 57 %N 1 %P 221-226 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-57-1-221 %K cell cycle analysis %K flow cytometry %K adenovirus 5 %K DNA content %I Microbiology Society, %X SUMMARY Human adenovirus type 5 caused acute hepatocellular damage when injected intravenously into C57B1/6 mice. Early protein (P) and late virus protein (V) antigen staining by immunofluorescence was located principally, if not exclusively, in hepatocytes. Autoradiography following incorporation of tritiated thymidine into Ad5-infected liver cells in vivo indicated that DNA synthesis was initiated in hepatocytes. Flow cytometry showed that cells with a 4n DNA content prior to infection were highly susceptible to infection, with 75% being lost from the liver cell population by 5 days post-infection. This observation was correlated with the observed pathology during the course of the infection. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-57-1-221