Murine hepatitis virus strains A59 and JHM replicated with equal efficiency in both nucleated and enucleated L2 cells. In addition, treatment of the host cell with either actinomycin D or µ-amanitin, both inhibitors of host cell RNA synthesis, had no effect on virus replication. Therefore, the replication of murine hepatitis virus did not appear to depend upon either the presence of the host cell nucleus or continued host cell RNA synthesis.
BondC. W.,
LeibowitzJ. L.,
RobbJ. A.1979; Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V. Virology 94:371–384
EvansM. R.,
SimpsonR. W.1980; The coronavirus avian infectious bronchitis virus requires the cell nucleus and host transcriptional factors. Virology 105:582–591
FollettE. A. C.,
PringleC. R.,
WunnerW. H.,
SkehelJ. J.1974; Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. Journal of Virology 13:394–399
LaiM. M. C.,
BraytonP. R.,
ArmenR. C.,
PattonC. D.,
StohlmanS. A.1981; Mouse hepatitis virus A59: messenger RNA structure and genetic localization of the sequence divergence from a hepatotropic strain MHV-3. Journal of Virology (in press)
MaziaD.,
SchattenG.,
SaleW.1975; Adhesion of cells to surfaces coated with polylysine: applications to electron microscopy. Journal of Cell Biology 66:198–200
RobbJ. A.,
BondC. W.1979; Pathogenic murine coronaviruses I. Characterization of biological behavior in vitro and virus-specific intracellular RNA of strongly neurotropic JHMV and weakly neurotropic A59V viruses. Virology 94:352–370
SpaanW. J. M.,
RottierP. J. M.,
HorzinekM. C.,
Van Der ZeustB. A. M.1981; Isolation and identification of virus-specific mRNAs in cells infected with mouse hepatitis virus (MHV-A59). Virology 108:424–434
SternD. F.,
KennedyS. I. T.1980; Coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA. Journal of Virology 34:665–674
StohlmanS. A.,
WeinerL. P.1978; Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cells. Archives of Virology 57:53–61