Intratypic electrophoretic mobility differences in high resolution SDS-polyacrylamide gels were detected between corresponding matrix (M) proteins, nucleoproteins (NP), haemagglutinin (HA) and the non-structural polypeptides NS1 and NS2 induced in Vero cells by human influenza A viruses of the antigenic subtypes H1N1 and H3N2. Such phenotypic differences were distinguishable in both H1N1 and H3N2 viruses isolated in single school and city outbreaks. Additional intratypic variation was detected in the biological property of virus plaquing in MDCK cells. Although the biochemical basis is not established, phenotypic variation could represent an additional factor influencing the epidemiology of influenza A viruses.
BrandC. M.,
StealyV. M.,
RoweJ.1977; Structural differences in matrix proteins of human influenza strains as revealed by 125I-labelled peptide maps. Journal of General Virology 36:385–394
ClevelandD. W.,
FischerS. G.,
KirschnerM. W.,
LaemmliU. K.1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
De JongW. W.,
ZweersA.,
CohenL. M.1978; Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulphate-protein complexes. Biochemical and Biophysical Research Communications 82:532–539
DimmockN. J.,
CarverA. S.,
WebsterR. G.1980; Categorization of nucleoprotein and matrix proteins from type A influenza viruses by peptide mapping. Virology 103:350–356
HenshawV. S.,
BeanW. J.,
WebsterR. G.,
EasterdayB. C.1978; The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine. Virology 89:51–62
InglisS. C.,
CarrollA. R.,
LambR. A.,
MahyB. W. J.1976; Polypeptides specified by the influenza virus genome 1. Evidence for eight distinct gene products specified by fowl plague virus. Virology 74:489–503
KendalA. P.,
JosephJ. M.,
KobayshiG.,
NelsonD.,
ReyesC. R.,
RossM. R.,
SarandriaJ. L.,
WhiteR.,
WoodaleD. F.,
NobelG. R.,
DowdleW. R.1979; Laboratory based surveillance of influenza virus in the United States during the winter of 1977–78. I. Periods of prevalence of H1N1 and H3N2 influenza strains, their relative rates of isolation in different age groups and detection of antigenic variants. American Journal of Epidemiology 110:449–461
RungH. C.,
JenK. F.,
YuanW. C.,
TienS. F.,
ChuC. M.1978; Influenza in China in 1977 recurrence of influenza A virus subtype (H IN 1). Bulletin of the World Health Organization 56:913–918
OxfordJ. S.,
McGeochD. J.,
SchildG. C.,
BeareA. S.1978; Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature, London 273:778–779
OxfordJ. S.,
SchildG. C.,
AlexandrovaG.1980a; Electrophoretic migration rate differences of polypeptides of human influenza A viruses: partial analysis of the genome of influenza vaccine recombinant viruses. Archives of Virology 65:277–291
OxfordJ. S.,
CorcoranT.,
SchildG. C.1980b; Naturally occurring is influenza A viruses of the H1N1 and H3N2 antigenic subtypes. Journal of General Virology 48:383–389
PaleseP.,
SchulmanJ. L.1976; RNA pattern of‘swine’ influenza virus isolated from man is similar to those of other swine influenza viruses. Nature, London 263:528–530
YoungJ. F.,
PaleseP.1979; Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H IN 1 strains. Proceedings of the National Academy of Sciences of the United States of America 76:6547–6551
YoungJ. F.,
DesselbergerV.,
PaleseP.1979; Evolution of human influenza A viruses in nature: sequential mutations in the genomes of new H1N1 isolates. Cell 18:73–83