1887

Abstract

SUMMARY

Intratypic electrophoretic mobility differences in high resolution SDS-polyacrylamide gels were detected between corresponding matrix (M) proteins, nucleoproteins (NP), haemagglutinin (HA) and the non-structural polypeptides NS1 and NS2 induced in Vero cells by human influenza A viruses of the antigenic subtypes H1N1 and H3N2. Such phenotypic differences were distinguishable in both H1N1 and H3N2 viruses isolated in single school and city outbreaks. Additional intratypic variation was detected in the biological property of virus plaquing in MDCK cells. Although the biochemical basis is not established, phenotypic variation could represent an additional factor influencing the epidemiology of influenza A viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-2-431
1981-10-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/2/JV0560020431.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-2-431&mimeType=html&fmt=ahah

References

  1. Bean W. J., Cox N. J., Kendal A. P. 1980; Recombination of human influenza A viruses in nature. Nature, London 284:638–640
    [Google Scholar]
  2. Brand C. M., Stealy V. M., Rowe J. 1977; Structural differences in matrix proteins of human influenza strains as revealed by 125I-labelled peptide maps. Journal of General Virology 36:385–394
    [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  4. De Jong W. W., Zweers A., Cohen L. M. 1978; Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulphate-protein complexes. Biochemical and Biophysical Research Communications 82:532–539
    [Google Scholar]
  5. Dimmock N. J., Carver A. S., Webster R. G. 1980; Categorization of nucleoprotein and matrix proteins from type A influenza viruses by peptide mapping. Virology 103:350–356
    [Google Scholar]
  6. Henshaw V. S., Bean W. J., Webster R. G., Easterday B. C. 1978; The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine. Virology 89:51–62
    [Google Scholar]
  7. Inglis S. C., Carroll A. R., Lamb R. A., Mahy B. W. J. 1976; Polypeptides specified by the influenza virus genome 1. Evidence for eight distinct gene products specified by fowl plague virus. Virology 74:489–503
    [Google Scholar]
  8. Kendal A. P., Joseph J. M., Kobayshi G., Nelson D., Reyes C. R., Ross M. R., Sarandria J. L., White R., Woodale D. F., Nobel G. R., Dowdle W. R. 1979; Laboratory based surveillance of influenza virus in the United States during the winter of 1977–78. I. Periods of prevalence of H1N1 and H3N2 influenza strains, their relative rates of isolation in different age groups and detection of antigenic variants. American Journal of Epidemiology 110:449–461
    [Google Scholar]
  9. Rung H. C., Jen K. F., Yuan W. C., Tien S. F., Chu C. M. 1978; Influenza in China in 1977 recurrence of influenza A virus subtype (H IN 1). Bulletin of the World Health Organization 56:913–918
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Lamb R. A., Etkind P. R., Choppin P. W. 1978; Evidence for a ninth viral polypeptide. Virology 91:60–78
    [Google Scholar]
  12. Laver W. G., Downie J. C. 1976; Influenza virus recombination. I. Matrix protein markers and segregation during mixed infections. Virology 70:105–117
    [Google Scholar]
  13. Oxford J. S., McGeoch D. J., Schild G. C., Beare A. S. 1978; Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature, London 273:778–779
    [Google Scholar]
  14. Oxford J. S., Schild G. C., Alexandrova G. 1980a; Electrophoretic migration rate differences of polypeptides of human influenza A viruses: partial analysis of the genome of influenza vaccine recombinant viruses. Archives of Virology 65:277–291
    [Google Scholar]
  15. Oxford J. S., Corcoran T., Schild G. C. 1980b; Naturally occurring is influenza A viruses of the H1N1 and H3N2 antigenic subtypes. Journal of General Virology 48:383–389
    [Google Scholar]
  16. Palese P., Schulman J. L. 1976; RNA pattern of‘swine’ influenza virus isolated from man is similar to those of other swine influenza viruses. Nature, London 263:528–530
    [Google Scholar]
  17. Ritchey M. B., Palese P., Schulman J. L. 1977; Differences in protein patterns of influenza A viruses. Virology 76:122–128
    [Google Scholar]
  18. Skehel J. J. 1972; Polypeptide synthesis in influenza virus infected cells. Virology 49:23–36
    [Google Scholar]
  19. Young J. F., Palese P. 1979; Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H IN 1 strains. Proceedings of the National Academy of Sciences of the United States of America 76:6547–6551
    [Google Scholar]
  20. Young J. F., Desselberger V., Palese P. 1979; Evolution of human influenza A viruses in nature: sequential mutations in the genomes of new H1N1 isolates. Cell 18:73–83
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-56-2-431
Loading
/content/journal/jgv/10.1099/0022-1317-56-2-431
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error