Diffraction Studies of Tulip Virus X Particles Free

Abstract

SUMMARY

X-ray diffraction from oriented tulip virus X (TVX) particles and optical diffraction from electron micrographs show that the virus particles have a helical structure with a pitch of about 3.25 nm and a true repeat in five turns of the helix. Both X-ray and optical diffraction indicate that the number of subunits in the repeat period is 5q + 4, where q is an integer. The X-ray diffraction patterns suggest that 9 ⩾ q ⩾ 6, and the optical diffraction patterns suggest that q is probably 7 or 8. The X-ray diffraction patterns indicate that there is a marked feature in the virus particle at a radial position of about 3.3 nm, which is probably the RNA. The equatorial diffraction further suggests that the virus has an axial hole of about 1.5 nm radius.

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/content/journal/jgv/10.1099/0022-1317-56-2-297
1981-10-01
2024-03-28
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References

  1. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435–449
    [Google Scholar]
  2. Bernal J. D., Fankuchen I. 1941; X-ray and crystallographic studies of plant virus preparations. Journal of General Physiology 25:111–165
    [Google Scholar]
  3. Franklin R. E., Holmes K. C. 1958; Tobacco mosaic virus: application of the method of isomorphous replacement to the determination of the helical parameters and radial density distribution. Acta Crystallographica 11:213–220
    [Google Scholar]
  4. Klug A., Berger J. E. 1964; An optical method for the analysis of periodicities in electronmicrographs and some observations on the mechanism of negative staining. Journal of Molecular Biology 10:565–569
    [Google Scholar]
  5. Koenig R. 1978; Potexvirus group. Commonwealth Mycological Institute!Association of Applied Biologists Descriptions of Plant Viruses No. 200
    [Google Scholar]
  6. Langridge R., Wilson H. R., Hooper C. W., Wilkins M. H. F., Hamilton L. D. 1960; The molecular configuration of deoxyribonucleic acid. I. X-ray diffraction study of a crystalline form of the lithium salt. Journal of Molecular Biology 2:19–37
    [Google Scholar]
  7. Mowat W. P. 1979 Report of the Scottish Horticultural Research Institute for 1978 p 102
    [Google Scholar]
  8. Richardson J. F., Tollin P., Bancroft J. B. 1981; The architecture of the Potexviruses. Virology 112:34–39
    [Google Scholar]
  9. Taylor C. A., Lipson H. 1964 Optical Transforms: Their Preparation and Application to X-ray Diffraction Problems London: G. Bell & Sons;
    [Google Scholar]
  10. Tollin P., Wilson H. R., Young D. W. 1968; X-ray diffraction evidence of the helical structure of narcissus mosaic virus. Journal of Molecular Biology 34:189–192
    [Google Scholar]
  11. Tollin P., Wilson H. R., Mowat W. P. 1975; Optical diffraction from particles of narcissus mosaic virus. Journal of General Virology 29:331–337
    [Google Scholar]
  12. Tollin P., Wilson H. R., Bancroft J. B. 1980; Further observations on the structure of potato virus X. Journal of General Virology 49:407–410
    [Google Scholar]
  13. Varma A., Gibbs A. J., Woods R. D., Finch J. T. 1968; Some observations on the structure of the filamentous particles of several plant viruses. Journal of General Virology 2:107–114
    [Google Scholar]
  14. Wilson H. R., Tollin P. 1969; Some observations on the structure of potato virus X. Journal of General Virology 5:151–154
    [Google Scholar]
  15. Wilson H. R., Tollin P., Rahman A. 1973; The structure of narcissus mosaic virus. Journal of General Virology 18:181–187
    [Google Scholar]
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