6-Resistant Phage-producing Mutants of Free

Abstract

SUMMARY

6 mutants of HB10Y were isolated and 12 out of 129 spontaneous mutants were found to produce phages during cellular growth. Following mitomycin C treatment 39 out of 82 isolated resistant mutants were phage-producing ones. The decrease in growth rate of these mutants corresponded roughly to the number of phages liberated into the medium. Prolonged storage of the mutants resulted in loss of production which could be regained by growing them with a high multiplicity of phages. The phage production phenomenon was independent of phage adsorption since both adsorbing and non-adsorbing phage-producing mutants were found. Occasionally, the phage-producing strains showed abnormally high numbers of intracellular phage particles in sectioned material. The producers were found to be 2 to 5 times more resistant than the normal host or non-producing 6 strains to the lytic enzyme associated with the 6 virion, indicating that they had an altered cell wall structure. The lytic enzyme of 6, needed both in penetration and progeny release, was less active on the altered cell wall, leading to diminished infection efficiency and deficient plaque formation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-2-287
1981-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/2/JV0560020287.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-2-287&mimeType=html&fmt=ahah

References

  1. Adams M. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Bamford D. H., Lounatmaa K. 1978; Freeze-fracturing of Pseudomonas phaseolicola infected by the lipid-containing bacteriophage ϕ6. Journal of General Virology 39:161–170
    [Google Scholar]
  3. Bamford D. H., Mindich L. 1980; Electron microscopy of cells infected with nonsense mutants of bacteriophage 06. Virology 107:222–228
    [Google Scholar]
  4. Bamford D. H., Palva E. T. 1980; Structure of the lipid-containing bacteriophage 06: distribution by Triton X-100 treatment. Biochimica et Biophysica Acta 601:245–259
    [Google Scholar]
  5. Bamford D. H., Palva E. T., Lounatmaa K. 1976; Ultrastructure and life cycle of the lipid-containing bacteriophage ϕ 6. Journal of General Virology 32:249–259
    [Google Scholar]
  6. Cuppels D. A., Vidaver A. K., Van Etten J. L. 1979; Resistance to bacteriophage ϕ6 by Pseudomonas phaseolicola. Journal of General Virology 44:493–504
    [Google Scholar]
  7. Davern C. I. 1964; The isolation and characterisation of an RNA bacteriophage. Australian Journal of Biological Science 17:719–725
    [Google Scholar]
  8. Fraser D. K. 1957; Host range mutants and semitemperate mutants of bacteriophage T3. Virology 3:527–533
    [Google Scholar]
  9. Hayes W. 1968 The Genetics of Bacteria and their Viruses 2nd edition pp 447–450 Oxford: Blackwell;
    [Google Scholar]
  10. Iba H., Nanno N., Okada Y. 1979; Identification and partial purification of a lytic enzyme in the bacteriophage < 56 virion. FEBS Letters 103:234–237
    [Google Scholar]
  11. Kakitani H., Emori Y., Iba H., Okada Y. 1978; Lytic enzyme activity associated with double-stranded RNA bacteriophage 06. Proceedings of the Japan Academy 54:337–340
    [Google Scholar]
  12. Kakitani H., Iba H., Okada Y. 1980; Penetration and partial uncoating of bacteriophage (56 particle). Virology 101:475–483
    [Google Scholar]
  13. Koibong L. I., Barksdale L., Garmise L. 1961; Phenotypic alterations associated with the bacteriophage carrier state of Shigella dysenteriae. Journal of General Microbiology 24:355–366
    [Google Scholar]
  14. Meynell E., Datta N. 1966; The nature and incidence of conjugation factors in Escherichia coli. Genetical Research 7:141–148
    [Google Scholar]
  15. Mindich L., Lehman I. 1979; Cell wall lysin as a component of the bacteriophage 06 virion. Journal of Virology 30:489–496
    [Google Scholar]
  16. Mindich L., Cohen J., Weisburd M. 1976; Isolation of nonsense suppressor mutants in Pseudomonas. Journal of Bacteriology 126:177–182
    [Google Scholar]
  17. Semancik J. S., Vidaver A. K., Van Etten J. L. 1973; Characterization of a segmented double-helical RNA from bacteriophage 06. Journal of Molecular Biology 78:617–625
    [Google Scholar]
  18. Sinclair J. F., Tzagoloff A., Levine D., Mindich L. 1975; Proteins of bacteriophage 06. Journal of Virology 16:685–695
    [Google Scholar]
  19. Stanisich V. 1974; The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. Journal of General Microbiology 84:322–342
    [Google Scholar]
  20. Tikkanen L., Bamford D. H. 1978; Lipid-containing double-stranded bacteriophage 06 resistant mutants of Pseudomonas phaseolicola. Abstracts of the XIV International Congress of Genetics Moscow Part 1 p 105
    [Google Scholar]
  21. Van Etten J., Lane L., Gonzalez C., Partridge J., Vidaver A. 1976; Comparative properties of bacteriophage 06 and 06 nucleocapsid. Journal of Virology 18:652–658
    [Google Scholar]
  22. Vidaver A. K., Koski R. K., Van Etten J. L. 1973; Bacteriophage 06: a lipid-containing virus of Pseudomonas phaseolicola. Journal of Virology 11:799–805
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-56-2-287
Loading
/content/journal/jgv/10.1099/0022-1317-56-2-287
Loading

Data & Media loading...

Most cited Most Cited RSS feed